|
| Status |
Public on Feb 05, 2013 |
| Title |
101_A05_2_bronchial_epithelium_asthma_subject |
| Sample type |
RNA |
| |
|
| Source name |
bronchial_epithelium_asthma_subject
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
subject group: patients with asthma
tissue: bronchial epithelium
cell type: isolated and cultured bronchial epithelial cells
condition: asthma
paired samples: 13
|
| Treatment protocol |
Cells were cultured to 80% confluence and were pre-incubated with BEBM for 48 hours prior to the removement of supernatant and RNA extraction
|
| Growth protocol |
Isolated airway epithelial cells were grown in fully humidified air containing 5% CO2 at 37°C, and culture medium was replaced every other day.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
According to standard Affymetrix protocol.
|
| Scan protocol |
According to standard Affymetrix protocol.
|
| Description |
101_A05_2
|
| Data processing |
Array images were analyzed by Affymetrix Expression Console using the robust multichip analysis (RMA) algorithm. The normalized data were further analysed using R (version 2.9) and the Bioconductor Limma package (v.3.14.1). Probesets without a known geneID were excluded for further analyses.
The data was further normalized in R by quantile normalization between the arrays
|
| |
|
| Submission date |
Feb 04, 2013 |
| Last update date |
Feb 05, 2013 |
| Contact name |
Ariane Wagener |
| E-mail(s) |
A.H.Wagener@amc.uva.nl
|
| Organization name |
Academic Medical Center
|
| Street address |
Meibergdreef 9
|
| City |
Amsterdam |
| ZIP/Postal code |
1015 AZ |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL13158 |
| Series (1) |
| GSE44037 |
Expression data from airway epithelial cells from patients with asthma, rhinitis, and helathy controls |
|
