|
| Status |
Public on Dec 31, 2013 |
| Title |
SPN-14 [miRNA] |
| Sample type |
RNA |
| |
|
| Source name |
ductal adenocarcinoma
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
age: 30
tissue: ductal adenocarcinoma (PCA)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was obtained from each independent tissue of patient and extracted using TRIzol Reagent (Invitrogen).
|
| Label |
Cy3-pCp
|
| Label protocol |
100ng total RNA was dephosphorylated at the presence of calf intestine alkaline phsphatase, then a Cy3-pCp molecule was then ligated to the 3' end of the RNA molecule according to Agilent miRNA protocol version 1.0
|
| |
|
| Hybridization protocol |
Human microRNA Microarray Kit (Agilent Technologies) according to manufacture’s protocol. (http://www.chem.agilent.com/en-US/Pages/Homepage.aspx)
|
| Scan protocol |
Scanned on an Agilent C scanner. Images were quantified using Agilent Feature Extraction Software ( v 10.10.1.1).
|
| Description |
S-14
|
| Data processing |
Raw data were extracted using the Agilent Feature Extraction Software (v 10.7.1.1) and then processed as published previously <Processing of Agilent microRNA array data, BMC RES NOTES,2010>
|
| |
|
| Submission date |
Jan 28, 2013 |
| Last update date |
Dec 31, 2013 |
| Contact name |
minhee park |
| Organization name |
student
|
| Street address |
shinchondong
|
| City |
seoul |
| ZIP/Postal code |
120-752 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL15159 |
| Series (2) |
| GSE43796 |
microRNA expression of pancreatic tumors |
| GSE43797 |
Characterization of mRNA and microRNA expression profiles in solid-pseudopapillary neoplasm of pancreas, ductal adenocarcinoma and pancreatic neuroendocrine tumors |
|
