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Sample GSM1071388 Query DataSets for GSM1071388
Status Public on Dec 31, 2013
Title SPN-2 [miRNA]
Sample type RNA
 
Source name solid-pseudopapillary neoplasm
Organism Homo sapiens
Characteristics gender: male
age: 30
tissue: Solid-pseudopapillary neoplasm of pancreas (SPN)
Extracted molecule total RNA
Extraction protocol Total RNA was obtained from each independent tissue of patient and extracted using TRIzol Reagent (Invitrogen).
Label Cy3-pCp
Label protocol 100ng total RNA was dephosphorylated at the presence of calf intestine alkaline phsphatase, then a Cy3-pCp molecule was then ligated to the 3' end of the RNA molecule according to Agilent miRNA protocol version 1.0
 
Hybridization protocol Human microRNA Microarray Kit (Agilent Technologies) according to manufacture’s protocol. (http://www.chem.agilent.com/en-US/Pages/Homepage.aspx)
Scan protocol Scanned on an Agilent C scanner. Images were quantified using Agilent Feature Extraction Software ( v 10.10.1.1).
Description S-2
Data processing Raw data were extracted using the Agilent Feature Extraction Software (v 10.7.1.1) and then processed as published previously <Processing of Agilent microRNA array data, BMC RES NOTES,2010>
 
Submission date Jan 28, 2013
Last update date Dec 31, 2013
Contact name minhee park
Organization name student
Street address shinchondong
City seoul
ZIP/Postal code 120-752
Country South Korea
 
Platform ID GPL15159
Series (2)
GSE43796 microRNA expression of pancreatic tumors
GSE43797 Characterization of mRNA and microRNA expression profiles in solid-pseudopapillary neoplasm of pancreas, ductal adenocarcinoma and pancreatic neuroendocrine tumors

Data table header descriptions
ID_REF
VALUE The value was log2 transformed and quantile normalized

Data table
ID_REF VALUE
A_25_P00010019 5.700461181
A_25_P00010020 5.677388054
A_25_P00010021 5.665720287
A_25_P00010023 5.777904158
A_25_P00010041 5.669330991
A_25_P00010042 5.6256693
A_25_P00010043 5.589376454
A_25_P00010044 5.533673691
A_25_P00010047 9.955866381
A_25_P00010048 8.347230028
A_25_P00010053 13.79594907
A_25_P00010054 12.05926529
A_25_P00010062 5.692317182
A_25_P00010063 5.648265238
A_25_P00010070 13.95111231
A_25_P00010071 13.56010282
A_25_P00010072 13.20357724
A_25_P00010073 11.6258197
A_25_P00010078 8.872595794
A_25_P00010079 7.416535021

Total number of rows: 3523

Table truncated, full table size 92 Kbytes.




Supplementary file Size Download File type/resource
GSM1071388_S-2.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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