|
Status |
Public on Dec 31, 2013 |
Title |
SPN-2 [miRNA] |
Sample type |
RNA |
|
|
Source name |
solid-pseudopapillary neoplasm
|
Organism |
Homo sapiens |
Characteristics |
gender: male age: 30 tissue: Solid-pseudopapillary neoplasm of pancreas (SPN)
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was obtained from each independent tissue of patient and extracted using TRIzol Reagent (Invitrogen).
|
Label |
Cy3-pCp
|
Label protocol |
100ng total RNA was dephosphorylated at the presence of calf intestine alkaline phsphatase, then a Cy3-pCp molecule was then ligated to the 3' end of the RNA molecule according to Agilent miRNA protocol version 1.0
|
|
|
Hybridization protocol |
Human microRNA Microarray Kit (Agilent Technologies) according to manufacture’s protocol. (http://www.chem.agilent.com/en-US/Pages/Homepage.aspx)
|
Scan protocol |
Scanned on an Agilent C scanner. Images were quantified using Agilent Feature Extraction Software ( v 10.10.1.1).
|
Description |
S-2
|
Data processing |
Raw data were extracted using the Agilent Feature Extraction Software (v 10.7.1.1) and then processed as published previously <Processing of Agilent microRNA array data, BMC RES NOTES,2010>
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|
|
Submission date |
Jan 28, 2013 |
Last update date |
Dec 31, 2013 |
Contact name |
minhee park |
Organization name |
student
|
Street address |
shinchondong
|
City |
seoul |
ZIP/Postal code |
120-752 |
Country |
South Korea |
|
|
Platform ID |
GPL15159 |
Series (2) |
GSE43796 |
microRNA expression of pancreatic tumors |
GSE43797 |
Characterization of mRNA and microRNA expression profiles in solid-pseudopapillary neoplasm of pancreas, ductal adenocarcinoma and pancreatic neuroendocrine tumors |
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