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Status
Public on Aug 11, 2006
Title
57_5dPA_MK02X023
Sample type
RNA
Source name
amputation blastema-proximal (1mm) tissue from Xenopus laevis hindlimbs
Organism
Xenopus laevis Characteristics
Stage 57 Xenopus leavis hindlimbs, 5 day post-amputation
Extracted molecule
total RNA
Label
biotin
Description
Gene expression data from Hindlimbs at either st57 were amputated unilaterally or bilaterally at the mid-zeugopodia level. Five days (5dPA) after amputation tissues were collected 1mm proximal to the original level of amputation. Total RNA was isolated using RNaqueous micro kit (Ambion, inc.).
Data processing
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Submission date
Apr 28, 2006
Last update date
Aug 10, 2006
Contact name
Matthew Grow
Organization name
Indiana University
Department
Biochemistry and Molecular Biology
Lab
Center for Medical Genomics
Street address
1345 W 16th St
City
Indianapolis
State/province
IN
ZIP/Postal code
46202
Country
USA
Platform ID
GPL1318 Series (1)
GSE4738 Global analysis of gene expression in Xenopus hindlimbs during stage-dependent complete and incomplete regeneration
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
689.5
P
0.000754
AFFX-BioB-M_at
840.7
P
0.000754
AFFX-BioB-3_at
504.7
P
0.000044
AFFX-BioC-5_at
1724.2
P
0.00007
AFFX-BioC-3_at
1639.6
P
0.000052
AFFX-BioDn-5_at
3496.1
P
0.000044
AFFX-BioDn-3_at
5289.1
P
0.000081
AFFX-CreX-5_at
16363.2
P
0.000044
AFFX-CreX-3_at
21484.1
P
0.000044
AFFX-DapX-5_at
21
A
0.5
AFFX-DapX-M_at
150.5
P
0.009337
AFFX-DapX-3_at
479.4
P
0.002275
AFFX-LysX-5_at
28.3
A
0.52976
AFFX-LysX-M_at
68.3
A
0.559354
AFFX-LysX-3_at
244.3
P
0.000581
AFFX-PheX-5_at
21.7
A
0.455409
AFFX-PheX-M_at
9.8
A
0.860518
AFFX-PheX-3_at
149.4
P
0.020022
AFFX-ThrX-5_at
9
A
0.976071
AFFX-ThrX-M_at
61.6
A
0.354453
15611 509 Kbytes .Supplementary data files not provided
