|
| Status |
Public on Feb 01, 2016 |
| Title |
HEK 293T-AntimiR rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Human embryonic kidney HEK 293T cells treated with 100nM AntimiR-195 for 24h
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Human Embryonic Kidney cell line
cell line: HEK 293T
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNA was extracted using TRIzol reagent (Invitrogen Corporation) according to the manufacturer's protocol.The RNA was resuspended in diethyl pyrocarbonate-treated water, quantified by UV absorbance at 260/280 nm and immediately used for RNA amplification and labeling by Illumina TotalPrep RNA amplification kit
|
| Label |
Cy3
|
| Label protocol |
As described in the illumina HumanHT12 V4.0 chip processing protocol. Briefly, generation of biotin labelled cRNA from the product of RT cDNA synthesis and 2nd strand cDNA synthesis.
|
| |
|
| Hybridization protocol |
Standard Illumina Hybridization protocol
|
| Scan protocol |
Refer to the Illumina Gene Expression System Manual
|
| Description |
This sample is hybridized to 5504654023_G and 5504654023_H arrays
|
| Data processing |
Average Normalization using Illumina Beadstudio
|
| |
|
| Submission date |
Jan 22, 2013 |
| Last update date |
Feb 01, 2016 |
| Contact name |
Richa Singh |
| E-mail(s) |
nsaini@igib.in
|
| Phone |
011-27666156
|
| Organization name |
Institute of Genomics and Integrative Biology
|
| Department |
Functional Genomics Unit
|
| Lab |
210
|
| Street address |
Mall Road
|
| City |
New Delhi |
| State/province |
Delhi |
| ZIP/Postal code |
110007 |
| Country |
India |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE43656 |
Differential expression of genes after alterations in cellular levels of hsa-miR-195 |
|
