cell line: LY7 cell type: diffuse large B-cell lymphoma (DLBCL) cells treatment: R406 time: 6h replicate: A
Treatment protocol
Each lymphoma cell line was profiled in triplicate at baseline (0h) and at two time points (6h, 24h) after treatment with R406. At 6 and 24 hours, cell lines were also profiled with vehicle alone (DMSO) to account for cell growth conditions.
Growth protocol
Five lymphoma cell lines, DHL4, DHL6, LY7, HBL1, and U2932, were propagated in their appropriate growth media under standard conditions.
Extracted molecule
total RNA
Extraction protocol
For total RNA, cells were homogenized in Trizol and RNA was extracted according to standard protocols.
Label
biotin
Label protocol
cRNA was biotinylated according to the standard Affymetrix protocol.
Hybridization protocol
Following fragmentation, cRNA was hybridized overnight according to the Affymetrix protocol.
Scan protocol
GCS3000 (Affymetrix).
Description
37_LY7_6h_R406_A Lymphoma cell line LY7 treated with R406 at 6h (replicate A).
Data processing
The raw CEL files were converted into probeset-specific expression values using the RMA summarization method as implemented in the BioConductor package simpleaffy (Gentleman et al., 2004).
Gene expression profiling of five diffuse large B-cell lymphoma (DLBCL) cell lines, DHL4, DHL6, LY7, HBL1 and U2932, treated with the SYK inhibitor, R406
