|
| Status |
Public on Jan 08, 2016 |
| Title |
Glioma patient 35 |
| Sample type |
RNA |
| |
|
| Source name |
Human brain tumor tissue from Glioma patient
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: glioma
gender: Female
age (years): 67
kps: 90
pathological diagnosis: GBM
who grade: 4
outcome: DEAD
survival time (days): 565
|
| Treatment protocol |
not applicable
|
| Growth protocol |
not applicable
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 1 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Expression arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100. The data is log2-transformed.
|
| |
|
| Submission date |
Jan 09, 2013 |
| Last update date |
Jan 08, 2016 |
| Contact name |
Ryuya Yamanaka |
| E-mail(s) |
ryaman@koto.kpu-m.ac.jp
|
| Organization name |
Kyoto Prefectural University of Medicine
|
| Lab |
Molecular target therapy for cancer
|
| Street address |
465 Kajii-cho, Kamigyoku
|
| City |
Kyoto |
| ZIP/Postal code |
602-8566 |
| Country |
Japan |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE43378 |
Expression data from glioma patients |
|
