|
| Status |
Public on Jan 01, 2013 |
| Title |
AK23_rest |
| Sample type |
RNA |
| |
|
| Source name |
skeletal muscle, resting
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
insulin sensitivity - m value: 10.3
tissue: skeletal muscle
exercise status: before exercise
|
| Treatment protocol |
volunteers had muscle biopsies at rest and 30 minutes after an exercise bout, and muscle biopsies were frozen in liquid nitrogen.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Muscle biopsy specimens were homogenized in RNAStat solution using a Polytron homogenizer, and the total RNA was purified with RNeasy and DNase I treatment.
|
| Label |
Cy3
|
| Label protocol |
Total RNA was amplified and labeled using the Amino Allyl MessageAmp II aRNA amplification kit and quantified for Cy3 dye labeled aRNA concentration, RNA absorbance ratio, A260, Adye, and dye/probe values.
|
| |
|
| Hybridization protocol |
Cy3-labeled aRNA was fragmented using Agilent Gene Expression Hybridization kit and hybidized to 4x44K Whole Human Genome Microarray at 65C for 17 hours
|
| Scan protocol |
After washing, slides were scanned with an Agilent DNA microarray scanner
|
| Description |
Gene expression before exercise
US92200302_251485047035_S01_GE1_105_Jan09_1_3
|
| Data processing |
Feature extraction software version 10.5.1.1 (Agilent) was used for analysis.
|
| |
|
| Submission date |
Dec 31, 2012 |
| Last update date |
Jan 01, 2013 |
| Contact name |
Dawn K Coletta |
| E-mail(s) |
dcoletta@deptofmed.arizona.edu
|
| Phone |
520-626-9316
|
| Organization name |
UNIVERSITY OF ARIZONA
|
| Department |
Medicine
|
| Street address |
1501 N. Campbell Ave
|
| City |
Tucson |
| State/province |
AZ |
| ZIP/Postal code |
85724 |
| Country |
USA |
| |
|
| Platform ID |
GPL4133 |
| Series (1) |
| GSE43219 |
mRNA changes induced by acute exercise |
|
