|
Status |
Public on Aug 05, 2013 |
Title |
DGM-10662_M0_sm |
Sample type |
RNA |
|
|
Source name |
small airway epithelium brushing
|
Organism |
Homo sapiens |
Characteristics |
smoking status: nonsmoker
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction and RNAeasy clean-up of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1-2 microg total RNA (Expression Analysis Technical Manual, 701022 Rev.2, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G.
|
Description |
gene expression
|
Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
|
|
Submission date |
Dec 20, 2012 |
Last update date |
Aug 05, 2013 |
Contact name |
Yael Strulovici-Barel |
E-mail(s) |
yas2003@med.cornell.edu
|
Organization name |
Weill Cornell Medical College
|
Department |
Department of Genetic Medicine
|
Lab |
Crystal
|
Street address |
1300 York Avenue
|
City |
New York |
State/province |
NY |
ZIP/Postal code |
10021 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (8)
|
GSE43079 |
Cigarette Smoking Induces Small Airway Epithelial Epigenetic Changes with Corresponding Modulation of Gene Expression |
GSE43939 |
Altered Expression of Intraflagellar Transport Genes is Associated with Short Cilia in Smoking and COPD |
GSE52237 |
Smoking accelerated aging of the small airway epithelium |
GSE63127 |
Cigarette smoking induces changes in airway epithelial expression of genes associated with monogenic lung disorders |
GSE76324 |
Role of OSGIN1 in Mediating Smoking-induced Autophagy in the Human Airway Epithelium [array] |
GSE76327 |
Role of OSGIN1 in Mediating Smoking-induced Autophagy in the Human Airway Epithelium |
GSE77658 |
POU2AF1 Functions in the Human Airway Epithelium to Regulate Expression of Host Defense Genes [array] |
GSE77659 |
POU2AF1 Functions in the Human Airway Epithelium to Regulate Expression of Host Defense Genes |
|