|
| Status |
Public on Apr 03, 2013 |
| Title |
ML-2 Human Cell Line Replicate 1 of 1 |
| Sample type |
RNA |
| |
|
| Source name |
ML2 human Leukemia cell line from DSMZ
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: ML-2
disease state: MLL-AF6 leukemia
|
| Treatment protocol |
Cells were not subject to any treatment prior to RNA preparation.
|
| Growth protocol |
The MLL-AF6 positive ML2 cell line (obtained from DSMZ) was grown in RPMI 10%FBS supplemented with 2mM L-glutamine and penicillin/streptomycin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA and Qiagen RNeasy micro kit was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
According to manufacturer's instructions (Affymetrix)
|
| Scan protocol |
According to manufacturer's instructions (Affymetrix)
|
| Description |
acute myelomonocytic cell line established from the peripheral blood of a 26-year-old man with acute myeloid leukemia (AML M4) at diagnosis of AML (following T-non-Hodgkin lymphoma and T-ALL) in 1978; carries t(6;11)(q27;q23) leading to MLL-MLLT4 (MLL-AF6) fusion gene
Gene expression data from a MLL-AF6 positive human cell line
|
| Data processing |
RMA background correction and quantile normalization done with the GenePattern ExpressionFileCreator module (http://www.broad.mit.edu/cancer/software/genepattern/) which uses the Bioconductor implementation of RMA (http://www.bioconductor.org/). This function computes the Robust Multichip Average expression measure described in Irizarry et al Biostatistics (2003).
|
| |
|
| Submission date |
Dec 20, 2012 |
| Last update date |
Apr 03, 2013 |
| Contact name |
Amit Sinha |
| E-mail(s) |
amit.sinha@childrens.harvard.edu
|
| Phone |
617-582-7579
|
| Organization name |
Dana-Farber Cancer Institute
|
| Department |
Pediatric Oncology
|
| Lab |
Armstrong Lab
|
| Street address |
44 Binney St
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02135 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE43068 |
Expression data from a human MLL-AF6 positive AML cell line |
| GSE43069 |
MLL-AF6 leukemia |
|
