|
| Status |
Public on Mar 01, 2013 |
| Title |
Malignant Breast Cell Line:BT549 |
| Sample type |
genomic |
| |
|
| Source name |
Malignant breast cell line, BaB/CLDNlow
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: BT549
cell type: malignant breast cell line
expression subtype: BaB/CLDNlow
er: 0
pr: 0
her2: 0
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted with TNES/PK (Tris/NaCl/EDTA/SDS/proteinase K) lysis buffer prior to sodium bisulfite conversion using the Zymo Research EZ DNA Methylation Kit.
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol for Infinium Methylation27 BeadChip.
|
| |
|
| Hybridization protocol |
Bisulphite-converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation27 BeadChips using the standard Illumina protocol.
|
| Scan protocol |
Arrays were imaged using Illumina iScan with Illumina scanner settings for the Infinium chip.
|
| Description |
BT549
0=negative; 1=positive
|
| Data processing |
GenomeStudio V2011.1. Beta=M/(M+U).
|
| |
|
| Submission date |
Dec 14, 2012 |
| Last update date |
Mar 01, 2013 |
| Contact name |
leslie cope |
| E-mail(s) |
cope@jhu.edu
|
| Organization name |
leslie cope
|
| Street address |
550 N. Broadway
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21209 |
| Country |
USA |
| |
|
| Platform ID |
GPL8490 |
| Series (1) |
| GSE42944 |
Genome-Wide Methylation Analysis of Breast Cancer Cell Lines |
|
