|
Status |
Public on Dec 01, 2013 |
Title |
Maver-1 2.5uM AEB071 3h rep1 |
Sample type |
RNA |
|
|
Source name |
Maver-1 cells
|
Organism |
Homo sapiens |
Characteristics |
mantle cell lymphoma (mcl) line: Maver-1 stn sensitivity: insensitive agent: 2.5uM Sotrastaurin
|
Treatment protocol |
3 million cells were resuspended in 3ml of fresh media in 6 well dishes. The next day, DMSO or Sotrastaurin was added for 3hrs.
|
Growth protocol |
Jeko-1 and Mino cells were cultured in 20% FBS RPMI; Z-138 and Maver-1 cells were cultured in 10% FBS RPMI.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNeasy Plus kit (qiagen) following the manufacturers instructions
|
Label |
biotin
|
Label protocol |
Nugen Ovation RNA Amplification System+ WB + FL cDNA Biotin Module V2 (NuGen Lts)
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|
|
Hybridization protocol |
Affymetrix GeneChip eukaryotic hybridization protocol (Affymetrix Inc.)
|
Scan protocol |
GeneChip Scanner 3000 (Affymetrix Inc.)
|
Description |
Gene expression data from Maver-1 cells treated with 2.5uM Sotrastaurin
|
Data processing |
Micorarray Analysis Suite version 5.0 (MAS 5.0) background correction, scaled to 150
|
|
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Submission date |
Nov 27, 2012 |
Last update date |
Dec 01, 2013 |
Contact name |
Joshua M Korn |
E-mail(s) |
joshkorn@gmail.com
|
Organization name |
Novartis
|
Street address |
250 Mass Ave
|
City |
Cambridge |
State/province |
MA |
ZIP/Postal code |
02141 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE42549 |
Transcriptional responses of mantle cell lymphoma (MCL) lines to PKC inhibition |
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