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Sample GSM104492 Query DataSets for GSM104492
Status Public on Sep 01, 2006
Title HUVEC/NmenMC58_pilD_6h_rep2_1107
Sample type RNA
Source name Human Umbilical Vein Endothelial Cells (HUVEC)
Organism Homo sapiens
Characteristics Tissue: endothelial cells
Biomaterial provider PromoCell Cat. no. C-12200-human umbilical vein endothelial cells; INSERM U570, Faculté de Médecine René Descartes, Paris, France, prof. Xavier Nassif - Neisseria meningitidis MC58
Treatment protocol Monolayers of HUVECs in 9 cm Petri dishes infected with Neisseria meningitidis MC58 delta pilD were washed and collected into Trizol by cell-scraper
Growth protocol Confluent monolayers of HUVECs in 9 cm Petri dishes were infected with Neisseria meningitidis MC58 delta pilD (MOI=10) and cultivated in RPMI medium, 5% CO2, humidified atmosphere, 37°C, 6 hours
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was purified on RNeasy minicolumns (Qiagen) and by NaOAc/ethanol precipitation.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Hybridization protocol Following fragmentation, cRNA was hybridized for 16 hr at 45C on Affymetrix Human Genome HG-U133A GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station.
Scan protocol GeneChips were scanned using the Agilent GeneArray scanner.
Description Gene expression data from HUVEC infected with Neisseria meningitidis MC58 delta pilD, 6 hours infection
Data processing The data were analyzed with RMA and MAS 5.0 algorithms using Biocondutor 1.7 and default analysis settings.
Submission date Apr 10, 2006
Last update date Apr 10, 2006
Contact name Marek Basler
Phone +420 2 4106 2762
Organization name Institute of Microbiology, Czech Academy of Sciences
Department Cell and Molecular Microbiology Division
Lab Laboratory of Molecular Biology of Bacterial Pathogens
Street address Videnska 1083
City Prague 4
ZIP/Postal code CZ-142 20
Country Czech Republic
Platform ID GPL96
Series (1)
GSE4646 Signaling of Neisseria meningitidis MC58 mutants to primary human umbilical vein endothelial cells (HUVEC)

Data table header descriptions
VALUE RMA-calculated Signal intensity
ABS_CALL indicates if the transcript was present (P), absent (A), marginal (M), based on MAS 5.0
DETECTION P-VALUE the significance level of the detection call, based on MAS 5.0
LOG2_VALUE Log2 transformed RMA-calculated Signal intensity, used for identification of significantly regulated genes

Data table
1007_s_at 142.6660032 M 0.043967773 7.156497777
1053_at 65.95417089 P 0.007542912 6.043391992
117_at 62.73372188 A 0.175989408 5.971169253
121_at 256.5029726 P 0.021866375 8.002831735
1255_g_at 13.61161061 A 0.162935019 3.766765881
1294_at 157.6653454 A 0.107301013 7.300721783
1316_at 20.43939463 P 0.00868917 4.353280562
1320_at 48.95172608 A 0.175989408 5.613287826
1405_i_at 15.25569204 A 0.774705591 3.931275721
1431_at 10.79316488 A 0.284747112 3.432046064
1438_at 101.4174228 A 0.541184238 6.664161709
1487_at 143.0519262 M 0.048994635 7.160395114
1494_f_at 117.9680009 P 0.039365362 6.882251768
1598_g_at 335.8808576 P 0.01309178 8.391805766
160020_at 445.0607097 P 0.003584915 8.797858334
1729_at 149.1624962 A 0.089405078 7.220741035
177_at 102.6468746 P 0.003584915 6.681545891
1773_at 67.93979681 A 0.127645309 6.086184998
179_at 603.9838665 A 0.089405078 9.238366203
1861_at 48.10378667 M 0.043967773 5.588078561

Total number of rows: 22283

Table truncated, full table size 1055 Kbytes.

Supplementary file Size Download File type/resource
GSM104492.CEL.gz 3.3 Mb (ftp)(http) CEL

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