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Status
Public on Jul 28, 2006
Title
Lung_high_2weeks_2h
Sample type
RNA
Channel 1 Source name
2-L1, lung tissue
Organism
Rattus norvegicus Characteristics
control
Extracted molecule
total RNA
Label
Cy3
Channel 2 Source name
2-L3, lung tissue
Organism
Rattus norvegicus Characteristics
rats exposed to high concentration of cigarette smoke, 2 weeks exposure, 2 hours recovery time after exposure
Extracted molecule
total RNA
Label
Cy5
Scan protocol
Image capture and signal quantification were done with the ScanArray Lite (Perkin Elmer, Boston, MA) and ImaGene software version 5.0 (BioDiscovery, Los Angeles, MA)
Description
2 µg of linear amplified RNA from cigarette smoke exposed and unexposed (control) rat tissue were labeled by reverse transcription with Cy5 and Cy3 fluorescence, respectively. Cy5 and Cy3 labeled cDNA were combined and subdivided into two parts to perform co-hybridizations with the arrays PIQOR 348 and PIQOR 350
Data processing
PubmedID
Submission date
Apr 08, 2006
Last update date
Jul 27, 2006
Contact name
Bernhard Gerstmayer
E-mail(s)
bernhard.gerstmayer@miltenyibiotec.de Phone
0049-221-95048211
Fax
0049-221-9504848
Organization name
Miltenyi Biotec GmbH
Street address
Stockheimer Weg 1
City
Cologne
ZIP/Postal code
50829
Country
Germany
Platform ID
GPL3607 Series (1)
GSE4644 The kinetics of transcriptomic changes induced by cigarette smoke in rat lungs
Data table header descriptions ID_REF VALUE normalized log2 ratio of means defined as Ch2/Ch1
Ch1I_Mean Channel 1 mean spot intensity
Ch1B_Mean Channel 1 mean background intensity
Ch2I_Mean Channel 2 mean spot intensity
Ch2B_Mean Channel 2 mean background intensity
Flag 0 denotes satisfactory feature while >0 denotes features that did not meet
Data table ID_REF VALUE Ch1I_Mean Ch1B_Mean Ch2I_Mean Ch2B_Mean Flag 1
-0.18
500.52
103.75
391.46
72.71
0
2
0.08
251.36
90.70
229.89
78.07
0
3
-0.10
46325.23
138.15
39001.99
86.73
0
4
-0.17
1275.04
103.99
994.53
80.99
0
5
-0.03
762.77
119.04
616.90
70.31
0
6
-0.29
1725.20
103.96
1241.15
77.00
0
7
0.26
366.82
94.53
341.96
72.77
0
8
-0.09
9101.46
110.48
7352.24
82.04
0
9
-0.30
30534.44
132.00
21579.39
96.75
0
10
0.04
963.92
93.33
817.44
72.58
0
11
0.11
13974.70
136.48
13936.10
84.65
0
12
-0.04
566.67
95.04
423.65
82.96
0
13
0.20
241.88
105.93
209.10
82.32
0
14
194.64
107.59
118.18
76.73
0
15
-0.30
236.46
91.42
178.76
76.29
0
16
-0.49
331.77
113.81
222.27
79.04
0
17
0.37
193.79
97.68
176.23
68.55
0
18
0.15
302.96
91.49
274.76
80.52
0
19
0.33
1306.40
102.68
1489.38
79.15
0
20
157.01
83.85
116.60
68.82
0
11994 448 Kbytes .Supplementary data files not provided
