|
| Status |
Public on Nov 20, 2014 |
| Title |
Miwi+/- early RS rep2 |
| Sample type |
SRA |
| |
|
| Source name |
early round spermtid
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Miwi+/-
cell type: early round spermatid
genetic background: mixed C57BL/6 and 129
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Trizol reagent from testes or FACS-purified cells.
Libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
Unstranded
|
| Data processing |
Read sequences were mapped to the genome with Tophat software, and reads that were uniquely and perfectly mapped to the genome were used for following analysis. RPKM values of Ensemble genes were calculated by using Cufflinks software.
Genome_build: mm9
Supplementary_files_format_and_content: [Gene ID] [Gene name] [Miwi+-rep1 RPKM] [Miwi+-rep2 RPKM] [Miwi+-rep3 RPKM] [Miwi--rep1 RPKM] [Miwi--rep2 RPKM] [Miwi--rep3 RPKM]
|
| |
|
| Submission date |
Nov 01, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Toshiaki Watanabe |
| E-mail(s) |
toshwatatoshiakiwatanabe@gmail.com
|
| Phone |
81-55-981-6796
|
| Organization name |
Yale University
|
| Street address |
10 Amistad
|
| City |
NewHaven |
| State/province |
CT |
| ZIP/Postal code |
06511 |
| Country |
USA |
| |
|
| Platform ID |
GPL13112 |
| Series (2) |
| GSE41974 |
Transcriptome analysis in mouse early round spermatids deficient in pachytene piRNAs (Miwi+/- and -/-) |
| GSE42004 |
Spermatogenesis |
|
| Relations |
| SRA |
SRX202281 |
| BioSample |
SAMN01797792 |
