|
Status |
Public on Mar 01, 2007 |
Title |
AML genome hybridization ID47-L |
Sample type |
genomic |
|
|
Source name |
ID47-L
|
Organism |
Homo sapiens |
Characteristics |
Leukemic fractions of ID47 AML
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was extracted by QIAamp DNA Mini Kit (Qiagen, Inc).
|
Label |
biotin
|
Label protocol |
Biotinylated genomic DNA were prepared according to the Affymetrix GeneChip Mapping 100K Assay protocol from 100 ug genomic DNA (GeneChip Mapping 100K Assay Manual, 2004, Affymetrix).
|
|
|
Hybridization protocol |
100 ug of genomic DNA were hybridized for 16 hr at 48C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
Description |
Leukemic fractions of ID47 AML patient have been hybridized.
|
Data processing |
The mean value for all probe sets was set to be 500.
|
|
|
Submission date |
Apr 04, 2006 |
Last update date |
Feb 26, 2007 |
Contact name |
Hiroyuki Mano |
E-mail(s) |
hmano@jichi.ac.jp
|
Phone |
+81-285-58-7449
|
Fax |
+81-285-44-7322
|
Organization name |
Jichi Medical University
|
Department |
Division of Functional Genomics
|
Street address |
3311-1 Yakushiji
|
City |
Shimotsukeshi |
State/province |
Tochigi |
ZIP/Postal code |
329-0498 |
Country |
Japan |
|
|
Platform ID |
GPL570 |
Series (2) |
GSE4602 |
AML genome hybridization to HGU133 Plus 2.0 |
GSE4610 |
AML hybridizations |
|