|
| Status |
Public on Jul 30, 2006 |
| Title |
WT 3.5dpf zebrafish muscle 2 |
| Sample type |
RNA |
| |
|
| Source name |
trunk and tail muscle, wildtype siblings 3.5dpf
|
| Organism |
Danio rerio |
| Characteristics |
Wildtype siblings of Runzel homozygotes on a TU/TL background (mix of heterozygous and homozygous WT)
3.5dpf
|
| Biomaterial provider |
Tuebingen
|
| Extracted molecule |
total RNA |
| Extraction protocol |
At 3-3.5 dpf (at the onset of the visible phenotype), runzel mutants (ruz) and wildtype siblings (WT) were quickly dissected to remove head, heart and gut from skeletal muscle of the trunk and tail. Pooled muscle was homogenized by douncing in Buffer RLT (Qiagen) and centrifuging through a QiaShredder column (Qiagen). RNA was extracted from homogenate using either the Qiagen RNeasy or RNeasy Micro kit with an on-column DNase step according to manufacturer instructions.
|
| Label |
Biotin
|
| Label protocol |
cRNA was produced by the Microarray Core Facility (Children's Hospital, Boston) and hybridized to Affymetrix Zebrafish Genome Arrays as previously described (Shepard et al., 2005).
|
| |
|
| Hybridization protocol |
cRNA was produced by the Microarray Core Facility (Children's Hospital, Boston) and hybridized to Affymetrix Zebrafish Genome Arrays as previously described (Shepard et al., 2005).
|
| Description |
Arrays were scanned in an Affymetrix/GeneChip Scanner 3000 and the resulting signals quantified and stored using default parameters of the Affymetrix GeneChip Operating Software Version 1.2. All data were in accordance with set limits provided by Affymetrix.
|
| Data processing |
Affymetrix default parameters
|
| |
|
| Submission date |
Mar 30, 2006 |
| Last update date |
Mar 31, 2006 |
| Contact name |
Leta S Steffen |
| E-mail(s) |
leta_steffen@student.hms.harvard.edu
|
| Organization name |
Harvard Medical School, Children's Hospital
|
| Department |
Program in Genomics
|
| Lab |
Louis M Kunkel
|
| Street address |
320 Longwood Ave
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL1319 |
| Series (1) |
| GSE4585 |
The zebrafish runzel mutant results in a novel muscular dystrophy phenotype due to a titin mutation |
|
