GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM101935 Query DataSets for GSM101935
Status Public on Mar 27, 2007
Title Xenograft - Scrambled - Rep 2
Sample type RNA
Source name Xenograft stable clone SA7 (scrambled)
Organism Homo sapiens
Characteristics Xenograft - human colon cancer cells (transfected with scrambled sequence) injected into male athymic mice,
tumors harvested on day 27,
clone SA7
Extracted molecule total RNA
Label biotin
Description We have found that NOX1 over-expression in colon cancer cell lines as well as surgical samples suggests a role in colon cancer. We identified unique target sequences of siRNAs for post-transcriptional silencing of NOX1. Gene specific and scrambled (control) sequences carrying the U6 promoter/shRNA cassettes were cloned into a GFP expression vector. Stable clones were selected based on NOX1 expression after transient transfection of HT-29 cells. HT-29 parental cells, clone SA (scrambled) and clone 6A (gene specific) were utilized to establish xenografts in athymic mice to investigate the effect of NOX1 silencing on tumor growth and gene expression in vivo. A significant decrease was measured in the volume of tumors formed. The stable clones and corresponding xenografts were analyzed on microarray. The microarray data was validated with real-time PCR and western-blot analysis demonstrating the inhibition of genes important in cell cycle regulation and angiogenesis. The results of this study show silencing NOX1 levels of generated ROS decreased, the tumor formation attenuated in vivo suggesting NOX1 may be a therapeutic target for colon cancer.
Data processing The following were applied using the affy library in the R-Project Bioconductor statistical tools package: RMA background correction, pmonly probe-level correction, quantile normalization, median-polish summary method.
Submission date Mar 27, 2006
Last update date Mar 29, 2006
Contact name Agnes Juhasz
Organization name City of Hope Compr. Cancer Center
Department Medical Oncology
Street address 1500 E. Duarte Rd
City Duarte
State/province CA
ZIP/Postal code 91010
Country USA
Platform ID GPL571
Series (1)
GSE4561 Inhibition of NOX1 Gene Expression with shRNA in Human Colon Cancer

Data table header descriptions

Data table
1007_s_at 10.89
1053_at 8.03
117_at 5.9
121_at 8.92
1255_g_at 4.13
1294_at 7.12
1316_at 6
1320_at 5.83
1405_i_at 4.03
1431_at 4.44
1438_at 7.73
1487_at 9.09
1494_f_at 5.84
1598_g_at 7.79
160020_at 7.58
1729_at 8.44
177_at 7.79
1773_at 6.37
179_at 9.21
1861_at 6.98

Total number of rows: 22277

Table truncated, full table size 346 Kbytes.

Supplementary file Size Download File type/resource
GSM101935.CEL.gz 3.4 Mb (ftp)(http) CEL

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap