|
| Status |
Public on Sep 25, 2012 |
| Title |
lung from control group, rep1 |
| Sample type |
RNA |
| |
|
| Source name |
lung from mock-infected mouse
|
| Organism |
Mus musculus |
| Characteristics |
strain: BALB/c
infection: control
tissue: lung
|
| Treatment protocol |
Lungs were collected from mice and immediately froze in liquid nitrogen and then stored at -70℃.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions. Qualified total RNA was further purified by Rneasy mini kit (QIAGEN,GmBH, Germany) and RNase-Free DNase Set (QIAGEN).
|
| Label |
Cy3
|
| Label protocol |
Total RNA was amplified and labeled by Low Input Quick Amp Labeling Kit,One-Color (Agilent technologies, Santa Clara, CA, US), followed the manufacturer’s instructions. Labeled cRNA were purified by RNeasy mini kit (QIAGEN).
|
| |
|
| Hybridization protocol |
Each Slide was hybridized with 1.65μg Cy3-labeled cRNA using Gene Expression Hybridization Kit (Agilent technologies) in Hybridization Oven (Agilent) according to the manufacturer’s instructions. After 17 hours hybridization, slides were washed in staining dishes (Thermo Shandon, Waltham, MA, US) with Gene Expression Wash Buffer Kit (Agilent).
|
| Scan protocol |
Slides were scanned by Agilent Microarray Scanner (Agilent) with default settings. Dye channel: Green, Scan resolution=5μm, PMT 100%, 10%, 16bit.
|
| Description |
Gene expression data of lung from mock-infected mouse
|
| Data processing |
Feature Extraction software 10.7 was used to analyzed data and raw data were normalized by Quantile algorithm Gene Spring Software 11.0.
|
| |
|
| Submission date |
Sep 25, 2012 |
| Last update date |
Sep 26, 2012 |
| Contact name |
Jiao Hu |
| E-mail(s) |
hujiao19844228@yahoo.com.cn
|
| Organization name |
yangzhou university
|
| Street address |
E. Wenhui Road 12
|
| City |
yangzhou |
| ZIP/Postal code |
225009 |
| Country |
China |
| |
|
| Platform ID |
GPL7202 |
| Series (1) |
| GSE41126 |
Expression data of lungs of mice infected with two highly pathogenic avian influenza viruses |
|
