|
| Status |
Public on Oct 12, 2013 |
| Title |
Tcell Roc A 36h |
| Sample type |
RNA |
| |
|
| Source name |
Tcell Roc A 36h
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: Roc-A 50nM
tissue: Blood
array id: 4675283019
|
| Treatment protocol |
Cells were plated in duplicates and treated for the indicated periods of time at 37 °C with solvent DMSO or 50 nM of Roc-A (>98% pure, assessed by HPLC) (BIOTREND Chemicals AG, Zurich, Swiss). Approximately 1 x 10P6P cells were collected and RNA was extracted as indicated.
|
| Growth protocol |
Healthy human peripheral blood T cells were prepared as described previously (Zhu et al., Int J Cancer, 2007) and were more than 90% CD3 positive. For generating proliferating T cells, freshly isolated peripheral blood T cells were cultured at a density of 2x10P6P cells/ml and were activated with 1 μg/ml PHA overnight. Activated T cells were then washed three times and cultured for additional 5 days in the presence of 25 U/ml IL-2. Jurkat-16 and peripheral blood T cells were cultured in RPMI 1640 medium supplemented with 10% FCS, 50 µg/ml gentamicin (GIBCO laboratories, Grand Island, NY).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with the QIAGEN RNeasy Plus Mini Kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Cy3
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Tcell RocA 36h
|
| Data processing |
All arrays were normalized together using the quantile normalization algorithm without background subtraction. The normalization has been done using Illumina's BeadStudio.
|
| |
|
| Submission date |
Sep 21, 2012 |
| Last update date |
Oct 12, 2013 |
| Contact name |
Hauke Busch |
| E-mail(s) |
hauke.busch@uni-luebeck.de
|
| Phone |
+49-451-3101-8470
|
| Organization name |
University of Lübeck
|
| Department |
Lübeck Institute of Experimental Dermatology
|
| Street address |
Ratzeburger Allee 160
|
| City |
Lübeck |
| State/province |
Schleswig-Holstein |
| ZIP/Postal code |
23538 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6883 |
| Series (1) |
| GSE41072 |
Rocaglamide selectively inhibits the G1-S-phase transition in cancer cells by activation of the ATM/ATR-mediated Chk1/2 cell cycle checkpoints |
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