|
| Status |
Public on Jun 16, 2015 |
| Title |
A2780-OCMI-P14 |
| Sample type |
RNA |
| |
|
| Source name |
SOC ovarian cancer cell line
|
| Organism |
Homo sapiens |
| Characteristics |
histology: Adenocarcinoma NOS
medium: OCMI
|
| Treatment protocol |
None
|
| Growth protocol |
Cells were grown in 10cm dishes and extracts were harvested at 80% confluency
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cell monolayers were lysed directly in RLT+ buffer and homogenized with Qiashredder columns (Qiagen Allprep kit).
|
| Label |
Biotin
|
| Label protocol |
Biotin-labeled cRNA were prepared with the Illumina TotalPrep RNA Amplivifation Kit (Ambion, Cat#IL1791)
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
SOC: standard-ovarian-cell line
|
| Data processing |
The microarray data were normalized using the quantile normalization method in the Linear Models for Microarray Data package in the R language environment
|
| |
|
| Submission date |
Sep 11, 2012 |
| Last update date |
Jun 16, 2015 |
| Contact name |
Tan A Ince |
| E-mail(s) |
Tince@med.miami.edu
|
| Phone |
305-243-1782
|
| Organization name |
University of Miami - Miller School of Medicine
|
| Department |
Sylvester Cancer Center
|
| Lab |
Dr. Ince Lab - TCC
|
| Street address |
1501 NW 10th Avenue, Rm 926
|
| City |
Miami |
| State/province |
FL |
| ZIP/Postal code |
33136 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE40785 |
Characterization of novel ovarian tumor cell lines that retain the phenotype of primary tumors [Illumina] |
| GSE40788 |
Characterization of novel ovarian tumor cell lines that retain the phenotype of primary tumors |
|
