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| Status |
Public on Nov 01, 2017 |
| Title |
Smad5 acts as an intracellular pH messenger and maintains bioenergetic homoeostasis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
we report here that Smad5 acts as a pHi messenger and maintains bioenergetic homeostasis of a cell by regulating cytoplasmic metabolic machinery
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| Overall design |
Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using a protocol from Chepelev et al., Nucleic Acids Research, 2009. In short, exons from all isoforms of a gene were merged to create one meta-transcript. The number of reads falling in the exons of this meta-transcript were counted and normalized by the size of the meta-transcript and by the size of the library.
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| Contributor(s) |
Fang Y, Liu Z, Chen Z, Xu X, Xiao M, Yu Y, Zhang Y, Zhang X, Du Y, Jiang C, Zhao Y, Wang Y, Fan B, Terheyden-Keighley D, Liu Y, Shi L, Hui Y, Zhang X, Zhang B, Feng H, Ma L, Zhang Q, Jin G, Yang Y, Xiang B, Liu L, Zhang X |
| Citation(s) |
28675158 |
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| Submission date |
Jun 05, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Xiaobai Zhang |
| E-mail(s) |
zhangxb@tongji.edu.cn
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| Organization name |
Tongji University
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| Street address |
1239 Siping Road
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| City |
Shanghai |
| ZIP/Postal code |
200092 |
| Country |
China |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA389303 |
| SRA |
SRP108628 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE99687_WT_LDN.id_rpkm.txt.gz |
500.4 Kb |
(ftp)(http) |
TXT |
| GSE99687_WT_SMAD5.id_rpkm.txt.gz |
518.8 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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