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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 31, 2017 |
| Title |
Reduced diploidization and survival improvement of semi-cloned mice produced from androgenetic haploid embryonic stem cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We found that the overall DNA methylation and hydroxymethylation in AG-haESCs are extremely low, and the downregulation of both de novo methyltransferase Dnmt3b and methylation maintenance enzyme Dnmt1was discovered responsible for this DNA hypomethylation. Further, our study discovered that the correction of DNA methylation can greatly reduce the incidence of diploidization and further improve the survival of semi-cloned mice produced from AG-haESCs.
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| Overall design |
Gene expression profiles by RNA-Seq were analyzed to investigate transcriptional changes between AG-haESCs and other samples.
Transcriptional profiling of 2i cultured ESC XX.
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| Contributor(s) |
He W, Zhang X, Xiong Z, Zhang Y, Zheng W, Hu X, Chen M, Huang H, Yang L, Kou X, Zhao Y, Yin J, Zhu Q, Jiang Y, Zhang L, Xu R, Tang Y, Li C, Wang H, Han Z, Jiang C, Chen J, Gao S |
| Citation(s) |
29396184 |
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| Submission date |
Jun 01, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Xiaobai Zhang |
| E-mail(s) |
zhangxb@tongji.edu.cn
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| Organization name |
Tongji University
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| Street address |
1239 Siping Road
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| City |
Shanghai |
| ZIP/Postal code |
200092 |
| Country |
China |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA388933 |
| SRA |
SRP108550 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE99588_RAW.tar |
280.0 Kb |
(http)(custom) |
TAR (of TXT) |
| GSE99588_gene_expression.txt.gz |
377.7 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
| Processed data provided as supplementary file |
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