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| Status |
Public on May 25, 2017 |
| Title |
MicroRNA-183 cluster continuously scales mechanical pain sensitivity by regulating basal and neuropathic pain gene pathways. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Nociception is protective and prevents tissue damage but can also facilitate chronic pain. If a general principle governs these two types of pain is unknown. Here, we show that both basal mechanical and neuropathic pain are controlled by microRNA-183 cluster in mice. This single cluster controls more than 80% of neuropathic pain-regulated genes and scales basal mechanical sensitivity and mechanical allodynia by regulating auxiliary voltage-gated calcium channel subunits a2d.
Basal sensitivity is controlled in nociceptors and allodynia involves TrkB+ light-touch mechanoreceptors. These light-touch sensitive neurons that normally do not elicit pain produce pain during neuropathy that is reversed by gabapentin. Thus, a single miRNA cluster continuously scales acute noxious mechanical sensitivity in nociceptive neurons and suppresses neuropathic pain transduction in a specific, light-touch sensitive neuronal type recruited during mechanical allodynia.
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| Overall design |
Bulk DRG RNA sequencing of control and tissue-specific knock-out animals, naive and after nerve injury
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| Contributor(s) |
Peng C, Ernfors P |
| Citation(s) |
28572455 |
| |
| Submission date |
May 24, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Patrik Ernfors |
| Organization name |
Karolinska Institutet
|
| Department |
Medical Biochemestry and Biophysics
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| Lab |
Molecular Neurobiology
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| Street address |
Solnavägen 9
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| City |
Stockholm |
| ZIP/Postal code |
17165 |
| Country |
Sweden |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA387751 |
| SRA |
SRP107957 |
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