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Series GSE99260 Query DataSets for GSE99260
Status Public on May 25, 2017
Title Menin controls the memory Th2 cell function by maintaining the epigenetic integrity of Th2 cells. [RNA-Seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Post-translational modifications of histones are well-established epigenetic modifications that play an important role in gene expression and regulation. These modifications are partly mediated by the Trithorax group (TrxG) complex, which regulates the induction or maintence of gene transcription. We investigated the role of Menin, a component of the TrxG complex, in the acquisition and maintenance of T helper type 2 (Th2) cell identity using T cell-specific Menin-deficient mice. Our gene expression analysis revealed that Menin was involved in the maintenance of the high level expression of the previously identified Th2-specific genes rather than the induction of these genes. This result suggests a role of Menin in the maintenance of Th2 cell identity. Menin directly bound to the Gata3 gene locus, and this Menin-Gata3 axis appeared to form a core unit of the Th2-specific gene regulatory network. Consistent with the phenotype of Menin-deficient Th2 cells observed in vitro, Menin deficiency resulted in the attenuation of effector Th2 cell-induced airway inflammation. In addition, in memory Th2 cells, Menin was found to play an important role in the maintenance of the expression of Th2-specific genes, including Gata3, Il4, and Il13. Consequently, Menin-deficient memory Th2 cells showed an impaired abiliy to recruit eosinophils to the lung, resulting in the attenuation of memory Th2 cell-induced airway inflammation. This study confirmed the critical role of Menin in Th2 cell-mediated immune responses.
Overall design Functions of Menin, a component of Trithorax group (TrxG) complex, are assessed by ChIP-seq and RNA-seq.
Contributor(s) Onodera A, Kiuchi M, Kokubo K, Kato M, Ogino T, Horiuchi S, Kanai U, Hirahara K, Nakayama T
Citation(s) 28659357
Submission date May 24, 2017
Last update date Jul 25, 2021
Contact name Atsushi Onodera
Organization name Chiba University
Department Immunology
Street address Chuo-ku Inohana 1-8-1
City Chiba
ZIP/Postal code 260-8670
Country Japan
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (4)
GSM2639659 Th2-1st_WT_RNA
GSM2639660 Th2-1st_MeninKO_RNA
GSM2639661 Th2-3rd_WT_RNA
This SubSeries is part of SuperSeries:
GSE99261 Menin controls the memory Th2 cell function by maintaining the epigenetic integrity of Th2 cells.
BioProject PRJNA388010
SRA SRP108067

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE99260_RAW.tar 550.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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