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| Status |
Public on Jul 07, 2017 |
| Title |
Transcriptional changes induced in human CD4+ cells upon ectopic expression of FOXP3 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Human CD4+CD45RA+CD25- cells were lentivirally transduced with wild-type or mutated (A384T or R397W) FOXP3, or an empty vector (EV). Transduced cells were sorted 14 days post-transduction based on GFP expression, and were restimulated with soluble anti-CD3 (30 ng/mL) and irradiated PBMCs (3x) for 14 more days. Cells were then activated with 0.5 μg/ml of phytohemagglutinin (PHA) in the presence or absence of SGF003 (8 μg/mL), and total RNA was extracted for microarray analysis. Overall, this study highlights the functional impact of TIP60 in FOXP3-driven Treg biology and provides a novel target for manipulation of human Treg activity.
This study analyzes the transcriptional changes induced by the ectopic expression of FOXP3 in human effector T cells. It also assesses the impact of the enhancement of TIP60/FOXP3 interaction using the TIP60 modifier SGF003
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| Overall design |
Total RNA obtained from FOXP3-transduced human CD4+CD25- cells
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| Contributor(s) |
Dhuban KB, Shao S, Piccirillo CA, Shao S |
| Citation(s) |
28783662 |
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| Submission date |
May 23, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Steven Shao |
| E-mail(s) |
steven.shao@mail.mcgill.ca
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| Organization name |
McGill University
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| Street address |
2155 Guy Street, suite 500
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| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H3H 2R9 |
| Country |
Canada |
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| Platforms (1) |
| GPL24324 |
[Clariom_S_Human_HT] Affymetrix Clariom S Assay HT, Human (Includes Pico Assay) |
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| Samples (16)
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| GSM2637894 |
Untreated A384T, biological rep2 |
| GSM2637895 |
SGF treated empty vector, biological rep1 |
| GSM2637896 |
SGF treated empty vector, biological rep2 |
| GSM2637897 |
Untreated vempty vector, biological rep1 |
| GSM2637898 |
Untreated vempty vector, biological rep2 |
| GSM2637899 |
SGF treated R397W, biological rep1 |
| GSM2637900 |
SGF treated R397W, biological rep2 |
| GSM2637901 |
Untreated R397W, biological rep1 |
| GSM2637902 |
Untreated R397W, biological rep2 |
| GSM2637903 |
SGF treated wild-type, biological rep1 |
| GSM2637904 |
SGF treated wild-type, biological rep2 |
| GSM2637905 |
Untreated wild-type, biological rep1 |
| GSM2637906 |
Untreated wild-type, biological rep2 |
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| Relations |
| BioProject |
PRJNA387627 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE99236_RAW.tar |
15.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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