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Series GSE99017 Query DataSets for GSE99017
Status Public on Sep 28, 2017
Title Temporal Control of Mammalian Cortical Neurogenesis by m6A Methylation
Organisms Homo sapiens; Mus musculus
Experiment type Other
Summary N6-methyladenosine (m6A), installed by the Mettl3/Mettl14 methyltransferase complex, is the most prevalent internal mRNA modification. Whether m6A regulates mammalian brain development is unknown. Here we show that Mettl14 deletion in the embryonic mouse brain diminishes m6A levels, prolongs cell cycle of radial glia cells, and extends cortical neurogenesis into postnatal stages. Mettl3 knockdown also prolongs neural progenitor cell cycle and promotes radial glia cell maintenance. m6A-sequencing of the embryonic mouse cortex reveals enrichment of mRNAs related to transcription factors, cell cycle and neuron differentiation, and Mettl14 deletion attenuates their decay. Notably, Mettl14-/- radial glia cells precociously express neuronal proteins. Further analysis uncovers previously unappreciated transcriptional pre-patterning in cortical neural stem cells. Comparison of m6A-mRNA landscapes between mouse and human cortical neural progenitors identifies human-specific tagging of transcripts related to epigenetic regulation and brain disorder risk genes. Our study reveals an epitranscriptomic mechanism in heightened transcriptional coordination during mammalian cortical neurogenesis.
Overall design m6A profiling of mouse developing brain (E13.5) (performed with 3 replicate pull-downs) and m6A profiling of human 47 day organoids (performed with 2 replicate pull-downs). In addition, there is m6A sequencing of PCW11 human brain cortex, performed with 3 replicate pull-downs
Citation(s) 28965759
Submission date May 17, 2017
Last update date Jul 25, 2021
Contact name Francisca Rojas Ringeling
Organization name Ludwig Maximilian University of Munich
Department Biochemistry
Lab Stefan Canzar
Street address Feodor-Lynen-Stra├če 25, 81377
City Munich
ZIP/Postal code 81377
Country Germany
Platforms (3)
GPL15520 Illumina MiSeq (Homo sapiens)
GPL16417 Illumina MiSeq (Mus musculus)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (15)
GSM2630319 mouse1_m6A
GSM2630320 mouse2_m6A
GSM2630321 mouse3_m6A
BioProject PRJNA387009
SRA SRP107200

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Supplementary file Size Download File type/resource
GSE99017_RAW.tar 2.0 Mb (http)(custom) TAR (of NARROWPEAK)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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