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Series GSE99006 Query DataSets for GSE99006
Status Public on Nov 16, 2018
Title Whole transcriptome profile of citrulline-specific B cells from patients with rheumatoid arthritis
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Although the contribution of B-cell derived autoreactive antibodies to rheumatoid arthritis (RA) has been studied extensively, the autoantibody-independent roles of B cells in the progression of the disease is not well-defined. By utilizing whole transcriptome profiling of human citrulline-specific B cells, we identified diverse inflammatory pathways, cytokines and transcriptional programs that define the biological role of B cells in RA and identify targets for drug intervention. Significantly, we identified the restricted expression of IL15Rα on citrulline-specific B cells and also demonstrated that B cells can express the epidermal growth factor amphiregulin (AREG) under inflammatory conditions. Mechanistically, we demonstrate that AREG has a direct impact on the cellular effectors of RA; it increases the proliferation and invasiveness of RA fibroblast-like synoviocytes (FLS) and synergizes with ACPA to enhance differentiation of osteoclasts. Overall, we present the first comprehensive transcriptome profile of autoreactive B-cell in an autoimmune disease, which can serve as a foundational dataset for studying the multi-faceted roles of B cells in autoimmune diseases
Overall design Whole transcriptome profile of CCP-specific B cells (RA-CCPPOS) with that of CCP-negative B cells (RA-CCPNEG) from the same donor and hemagglutinin-specific (HAPOS) B cells from healthy individuals elicited upon vaccination with seasonal influenza virus
Contributor(s) Varadarajan N, Mahendra A
Citation(s) 30407753
Submission date May 17, 2017
Last update date Feb 28, 2020
Contact name Navin Varadarajan
Organization name University of Houston
Department Chemical and Biomolecular Engineering
Street address 4726 Calhoun Rd
City Houston
State/province Texas
ZIP/Postal code 77204
Country USA
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (12)
BioProject PRJNA386964
SRA SRP107189

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Supplementary file Size Download File type/resource
GSE99006_RA_Normalized_counts.txt.gz 951.8 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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