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| Status |
Public on Nov 16, 2018 |
| Title |
Whole transcriptome profile of citrulline-specific B cells from patients with rheumatoid arthritis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Although the contribution of B-cell derived autoreactive antibodies to rheumatoid arthritis (RA) has been studied extensively, the autoantibody-independent roles of B cells in the progression of the disease is not well-defined. By utilizing whole transcriptome profiling of human citrulline-specific B cells, we identified diverse inflammatory pathways, cytokines and transcriptional programs that define the biological role of B cells in RA and identify targets for drug intervention. Significantly, we identified the restricted expression of IL15Rα on citrulline-specific B cells and also demonstrated that B cells can express the epidermal growth factor amphiregulin (AREG) under inflammatory conditions. Mechanistically, we demonstrate that AREG has a direct impact on the cellular effectors of RA; it increases the proliferation and invasiveness of RA fibroblast-like synoviocytes (FLS) and synergizes with ACPA to enhance differentiation of osteoclasts. Overall, we present the first comprehensive transcriptome profile of autoreactive B-cell in an autoimmune disease, which can serve as a foundational dataset for studying the multi-faceted roles of B cells in autoimmune diseases
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| Overall design |
Whole transcriptome profile of CCP-specific B cells (RA-CCPPOS) with that of CCP-negative B cells (RA-CCPNEG) from the same donor and hemagglutinin-specific (HAPOS) B cells from healthy individuals elicited upon vaccination with seasonal influenza virus
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| |
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| Contributor(s) |
Varadarajan N, Mahendra A |
| Citation(s) |
30407753 |
| Submission date |
May 17, 2017 |
| Last update date |
Feb 28, 2020 |
| Contact name |
Navin Varadarajan |
| Organization name |
University of Houston
|
| Department |
Chemical and Biomolecular Engineering
|
| Street address |
4726 Calhoun Rd
|
| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
77204 |
| Country |
USA |
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| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA386964 |
| SRA |
SRP107189 |
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