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Series GSE98856 Query DataSets for GSE98856
Status Public on Aug 21, 2017
Title N6-methyladenosine (m6A) recruits and repels proteins to regulate mRNA homeostasis
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary RNA modifications are integral to regulation of RNA metabolism. One such abundant mRNA modification is m6A, which impacts various aspects of RNA metabolism including splicing, transport and degradation. Current knowledge about proteins recruited to m6A to carry out these molecular processes is still limited. Here we describe a comprehensive and systematic mass spectrometry-based screening of m6A interactors in various cell types and species. Amongst the main findings, we identified G3BP1 as a protein, which is repelled by m6A and which positively regulates mRNA stability in an m6A regulated manner. Furthermore, we identified FMR1 as a novel, RNA sequence context dependent m6A reader, thus revealing a connection between an mRNA modification and an autism spectrum disorder. Collectively, our data represents a rich resource for the community and sheds further light on the complex interplay between m6A, m6A interactors and mRNA homeostasis.
 
Overall design Transcriptome wide profiling of G3BP1 and G3BP2 binding sites and mRNA half-live measurement after G3BP1 overexpression or knockdown.
 
Contributor(s) Edupuganti R, Vermeulen M
Citation(s) 28869609
Submission date May 12, 2017
Last update date May 15, 2019
Contact name Rik Lindeboom
Organization name Wellcome Sanger Institute
Department Cellular Genetics
Lab Teichmann Lab
Street address Wellcome Trust Genome Campus
City Hinxton
ZIP/Postal code CB10 1RQ
Country United Kingdom
 
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (25)
GSM2616802 HeLa_Con-0h_RNA-seq to determine mRNA half-lives
GSM2616803 HeLa_Con-3h_RNA-seq to determine mRNA half-lives
GSM2616804 HeLa_Con-6h_RNA-seq to determine mRNA half-lives
Relations
BioProject PRJNA386460
SRA SRP106954

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE98856_G3BP1_processed.xlsx 405.9 Kb (ftp)(http) XLSX
GSE98856_G3BP2_processed.xlsx 350.3 Kb (ftp)(http) XLSX
GSE98856_quantification_g3bp1KdG3bp1Oe.txt.gz 20.6 Mb (ftp)(http) TXT
GSE98856_quantification_g3bp1KdMettl3Kd.txt.gz 18.5 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record
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