|
| Status |
Public on Jan 15, 2018 |
| Title |
Loss of CNFy toxin-induced inflammation drives Yersinia pseudotuberculosis into persistency |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Gastrointestinal infections caused by enteric yersiniae can become persistent and complicated by relapsing enteritis and severe autoimmune disorders. To establish a persistent infection, the bacteria have to cope with hostile surroundings when they transmigrate through the intestinal epithelium and colonize underlying gut-associated lymphatic tissues. How the bacteria gain a foothold in the face of host immune responses is poorly understood. Here, we show that the CNFY toxin, which enhances translocation of the antiphagocytic Yop effectors, induces IL-6-triggered inflammatory responses. This results in extensive tissue destruction, alteration of the intestinal microbiota and bacterial clearance. An elimination of CNFY, however, increases inter-feron-γ-mediated responses, comprising non-inflammatory antimicrobial activities and Ido1-promoted tolerogenesis. This process is accompanied by a preterm reprogram-ming of the pathogen's transcriptional response towards persistence, which gives the bacteria a fitness edge against host responses. These results have significant implica-tions for our understanding how enteric bacteria establish a commensal-type life style.
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| |
|
| Overall design |
RNA of Y. pseudotuberculosis infected and uninfected caecal tissue of Mus musculus
|
| |
|
| Contributor(s) |
Beckstette M, Heine W, Dersch P, Heroven A |
| Citation(s) |
29390040 |
| |
| Submission date |
May 11, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Michael Beckstette |
| Organization name |
Helmholtz Centre for Infection Research
|
| Department |
Molecular Infection Biology
|
| Lab |
Experimental Immunology
|
| Street address |
Inhoffenstrase 7
|
| City |
Braunschweig |
| State/province |
Lower Saxony |
| ZIP/Postal code |
38124 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (18)
|
|
| Relations |
| BioProject |
PRJNA386276 |
| SRA |
SRP106863 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE98802_DESeq2_genes_diffexp_by_fc_42_dpi_deltacnfY_vs_42_dpi_YPIII.xls.gz |
4.3 Mb |
(ftp)(http) |
XLS |
| GSE98802_DESeq2_genes_diffexp_by_fc_42_dpi_uninfected_vs_42_dpi_YPIII.xls.gz |
4.6 Mb |
(ftp)(http) |
XLS |
| GSE98802_DESeq2_genes_diffexp_by_fc_42_dpi_uninfected_vs_42_dpi_deltacnfY.xls.gz |
5.2 Mb |
(ftp)(http) |
XLS |
| GSE98802_DESeq2_genes_diffexp_by_fc_5_dpi_YPIII_vs_5_dpi_deltacnfY.xls.gz |
4.3 Mb |
(ftp)(http) |
XLS |
| GSE98802_DESeq2_genes_diffexp_by_fc_5_dpi_uninfected_vs_5_dpi_YPIII.xls.gz |
4.9 Mb |
(ftp)(http) |
XLS |
| GSE98802_DESeq2_genes_diffexp_by_fc_5_dpi_uninfected_vs_5_dpi_deltacnfY.xls.gz |
4.9 Mb |
(ftp)(http) |
XLS |
| GSE98802_RAW.tar |
8.7 Mb |
(http)(custom) |
TAR (of TAB) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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