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Series GSE98731 Query DataSets for GSE98731
Status Public on Oct 05, 2018
Title Metformin reduces air pollution-induced thrombosis
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary As our results suggested that metformin acts to limit mitochondrial ROS and calcium-mediated activation of IL-6, we reasoned it would likely affect other processes in alveolar macrophages triggered by exposure to particulate matter (PM). Therefore, we treated mice with metformin in the drinking water for 24 hours before we instilled PM intratracheally. We then flow-sorted alveolar macrophages from whole lung homogenates 24 hours later for transcriptomic analysis (RNA-Seq).
 
Overall design 3-4 biological replicates per group were used.
 
Contributor(s) Soberanes S, Misharin AV, McQuattie-Pimentel AC, Mutlu GM, Budigner GS
Citation(s) 30318339
NIH grant(s)
Grant ID Grant title Affiliation Name
R56 HL135124 Differential role of tissue-resident and monocyte-derived alveolar macrophages in the development of pulmonary fibrosis NORTHWESTERN UNIVERSITY AT CHICAGO Alexander Misharin
R01 ES013995 Signaling in the lung induced by particulate matter air pollution NORTHWESTERN UNIVERSITY AT CHICAGO G R Scott Budinger
P01 HL071643 Murine Genetics and Phenotyping Core NORTHWESTERN UNIVERSITY AT CHICAGO G R Scott Budinger
P01 HL071643 Metabolic Regulation of Acute Lung Injury NORTHWESTERN UNIVERSITY AT CHICAGO NAVDEEP S CHANDEL
P01 AG049665 Disordered Proteostasis as a Driver of Disease in the Aging Lung NORTHWESTERN UNIVERSITY AT CHICAGO G R Scott Budinger
P01 AG049665 Modulating mitochondrial function to promote proteostasis in the aging lung NORTHWESTERN UNIVERSITY AT CHICAGO NAVDEEP S CHANDEL
I01 BX000201 The Role of Mitochondrial Reactive Oxygen Species in TGF-Beta Signaling JESSE BROWN VA MEDICAL CENTER G R Scott Budinger
I01 BX000201 The Role of Mitochondrial Reactive Oxygen Species in TGF-Beta Signaling JESSE BROWN VA MEDICAL CENTER MANU JAIN
Submission date May 09, 2017
Last update date May 15, 2019
Contact name Alexander V Misharin
E-mail(s) a-misharin@northwestern.edu
Phone 3125031958
Organization name Northwestern University
Department Pulmonary and Critical Care
Lab Misharin lab
Street address 240 E Huron
City Chicago
State/province IL
ZIP/Postal code 60611
Country USA
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (13)
GSM2610749 M21.AM.AcuteIT.Vehicle.PBS.1
GSM2610750 M22.AM.AcuteIT.Vehicle.PBS.2
GSM2610751 M23.AM.AcuteIT.Vehicle.PBS.3
Relations
BioProject PRJNA386043
SRA SRP106751

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE98731_htseq.all.counts.txt.gz 1017.9 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record
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