|
| Status |
Public on Jul 14, 2017 |
| Title |
Gene expression profiling of continuous growth hormone infused (cGH) adult male mouse liver by RNA-seq analysis of rRNA-depleted liver RNA. [G137_G138] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
rRNA-depleted RNA isolated from livers of intact male and female mice and from male mice treated with a continuous infusion of growth hormone for either 10 hr or 1 days was analyzed by RNA-seq
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| Overall design |
Liver RNA was isolated from 8 week old male mice treated with a continuous GH infusion (cGH) for either 10 hours or 1 day. Sham pump males served as a control. RNA-seq data are compared to untreated adult females to identify genes that show sex differences in liver expression and also respond to cGH. RNA samples were pooled to make 3 biological replicates per condition comprised of 2-4 individuals each.
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| |
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| Contributor(s) |
Lau-Corona D, Suvorov A, Waxman DJ |
| Citation(s) |
28694329 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 DK033765 |
Regulation of Sex Differences in Liver Metabolism |
BOSTON UNIVERSITY CHARLES RIVER CAMPUS |
DAVID J WAXMAN |
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| |
| Submission date |
May 05, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
David J. Waxman |
| E-mail(s) |
djw@bu.edu
|
| Organization name |
Boston University
|
| Department |
Department of Biology and Bioinformatics Program
|
| Street address |
5 Cummington Mall
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (12)
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| GSM2601318 |
GH infusion_10h male liver pool 1_rRNA-depleted_G137_M10 |
| GSM2601319 |
GH infusion_10h male liver pool 2_rRNA-depleted_G137_M11 |
| GSM2601320 |
GH infusion_10h male liver pool 3_rRNA-depleted_G137_M12 |
| GSM2601321 |
GH infusion_1d male liver pool 1_rRNA-depleted_G137_M13 |
| GSM2601322 |
GH infusion_1d male liver pool 2_rRNA-depleted_G137_M14 |
| GSM2601323 |
GH infusion_1d male liver pool 3_rRNA-depleted_G137_M15 |
| GSM2601324 |
Control male liver pool 1_rRNA-depleted_G138_M10 |
| GSM2601325 |
Control male liver pool 2_rRNA-depleted_G138_M11 |
| GSM2601326 |
Control male liver pool 3_rRNA-depleted_G138_M12 |
| GSM2601327 |
Intact female liver pool 1_rRNA-depleted_G138_M13 |
| GSM2601328 |
Intact female liver pool 2_rRNA-depleted_G138_M14 |
| GSM2601329 |
Intact female liver pool 3_rRNA-depleted_G138_M15 |
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| This SubSeries is part of SuperSeries: |
| GSE98586 |
Gene expression profiling of continuous growth hormone infused (cGH) adult male mouse liver by RNA-seq analysis of liver RNA. |
|
| Relations |
| BioProject |
PRJNA385602 |
| SRA |
SRP106531 |
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