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| Status |
Public on Feb 28, 2018 |
| Title |
A versatile approach for assessing human stem cell-derived retinal progeny using single cell transcriptomics |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To study photoreceptor (PR) production in human pluripotent stem cells (hPSCs), we created a reporter line that robustly labels PRs throughout differentiation. We then performed scRNAseq to define the molecular signature of hPSC-PRs
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| Overall design |
We employed both a genetically engineered reporter line and scRNAseq to examine PRs and other NR cell types in serum-free cultures of differentiating hPSC-Ovs (D070* and D218* samples). Adult* samples are from adult retinal tissue.
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| |
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| Contributor(s) |
Phillips MJ, Jiang P, Howden S |
| Citation(s) |
29230913 |
| |
| Submission date |
May 04, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Peng Jiang |
| E-mail(s) |
p.jiang@csuohio.edu
|
| Organization name |
Cleveland State University
|
| Street address |
2121 Euclid Ave
|
| City |
Cleveland |
| State/province |
OH |
| ZIP/Postal code |
44115 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (546)
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| Relations |
| BioProject |
PRJNA385507 |
| SRA |
SRP106476 |
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