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| Status |
Public on Oct 22, 2017 |
| Title |
Transcriptional effects of dominant-negative G784E Smarca4 mutation in mouse embryonic stem cells [RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We investigated transcriptomic effects of a Smarca4 ATPase mutant observed in primary tumors and cancer cell lines. Heterozygous expression of G784E Smarca4 mutant led to transcriptional changes compared to cells expressing only wild-type Smarca4.
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| Overall design |
Examination of transcriptome changes using RNA-seq in mouse embryonic stem (ES) cells expressing heterozygous mutations of the BAF subunit Smarca4. Cells express wild-type Smarca4-GFP and either wild-type or G784E Smarca4-V5. Filenames and descriptions of Smarca4 in this dataset refer to the status of the Smarca4-V5 fusion in these cells.
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| Contributor(s) |
Hodges C, Stanton BZ, Cermakova K, Chang C, Miller EL, Kirkland JG, Ku WL, Veverka V, Zhao K, Crabtree G |
| Citation(s) |
29323272 |
| |
| Submission date |
May 02, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
H. Courtney Hodges |
| Organization name |
Baylor College of Medicine
|
| Department |
Department of Molecular & Cellular Biology
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| Lab |
Hodges Lab
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| Street address |
1 Baylor Plaza, BCM 229
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE98605 |
Dominant-negative SMARCA4 mutants alter the accessibility landscape of tissue-unrestricted enhancers |
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| Relations |
| BioProject |
PRJNA385176 |
| SRA |
SRP106076 |
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