|
| Status |
Public on Dec 31, 2017 |
| Title |
Analysis of transcriptional differences between ZIKV-infected Ifnar-/- and Ifnar+/- placentas |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: While type I interferons (IFN) are important for control of viral replication, we see that, upon infection of Ifnar-/- females were crossed to Ifnar+/- males with ZIKV, only Ifnar+/- fetuses are resorbed or develop severe growth restriction. To identify how IFN may be altering placenta development, we performed RNAsequencing on infected and uninfected Ifnar-/- and Ifnar+/- placentas.
Methods: Ifnar-/- females were crossed to Ifnar+/- males and infected intravaginally with Cambodian ZIKV at E5.5. Placentas were harvested from infected and uninfected pregnant dams at E10.5.
Results/ Conclusions: Comparison of ZIKV-infected Ifnar-/- and Ifnar+/- shows an elevated interferon stimulated gene (ISG) signature in Ifnar+/- placentas but does not reveal any underlying developmental defects that may be causing defects in placenta development.
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| |
|
| Overall design |
MRNA profiles from 3 placentas per genotype from uninfected and 5 placentas per genotype of infected were generated using Illumina NextSeq 500.
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| |
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| Contributor(s) |
Iwasaki A, Yockey L |
| Citation(s) |
29305462 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R21 AI131284 |
Innate antiviral defense against Vaginal transmission of ZIKA virus |
YALE UNIVERSITY |
AKIKO IWASAKI |
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| |
| Submission date |
May 02, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Akiko Iwasaki |
| E-mail(s) |
akiko.iwasaki@yale.edu
|
| Organization name |
Yale
|
| Street address |
1 Gilbert St
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06511 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (16)
|
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| This SubSeries is part of SuperSeries: |
| GSE104349 |
Transcriptional analysis of ZIKV-infected Ifnar-/- and Ifnar+/- placentas and IFN-beta (IFN-b) or IFN-lambda (IFN-l) treated human mid-gestation chorionic villus explants |
|
| Relations |
| BioProject |
PRJNA385077 |
| SRA |
SRP106009 |
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