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| Status |
Public on Feb 15, 2018 |
| Title |
The AP-1 Transcription Factor JunB Is Required for Th17 Cell Differentiation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Interleukin (IL)-17-producing T helper (Th17) cells are crucial for host defense against extracellular microbes and pathogenesis of autoimmune diseases. Here we show that the AP-1 transcription factor JunB is required for Th17 cell development. Junb-deficient CD4+ T cells are able to develop in vitro into various helper T subsets except Th17. The RNA-seq transcriptome analysis reveals that JunB is crucial for the Th17-specific gene expression program. Junb-deficient mice are completely resistant to experimental autoimmune encephalomyelitis, a Th17-mediated inflammatory disease, and naive T helper cells from such mice fail to differentiate into Th17 cells. JunB appears to activate Th17 signature genes by forming a heterodimer with BATF, another AP-1 factor essential for Th17 differentiation. The mechanism whereby JunB controls Th17 cell development likely involves activation of the genes for the Th17 lineage-specifying orphan receptors RORt and ROR and reduced expression of Foxp3, a transcription factor known to antagonize RORt function.
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| Overall design |
Naïve T cell, Th0 and Th17 mRNA profiles of wild type (WT) and Junb-/- mice were generated by deep sequencing, using Illumina HiSeq2500
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| Contributor(s) |
Yamazaki S, Tanaka Y, Araki H, Kohda A, Sanematsu F, Arasaki T, Duan X, Miura F, Ito T, Fukui Y, Endo S, Sumimoto H |
| Citation(s) |
29234109 |
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| Submission date |
Apr 26, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Hiromitsu Araki |
| E-mail(s) |
araki.hiromitsu.596@m.kyushu-u.ac.jp
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| Organization name |
Kyushu University
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| Street address |
744 Motooka Nishi-Ku
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| City |
Fukuoka |
| ZIP/Postal code |
819-0395 |
| Country |
Japan |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA384377 |
| SRA |
SRP105275 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE98242_RAW.tar |
15.3 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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