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| Status |
Public on Jun 19, 2018 |
| Title |
Evaluation of functional properties of current and novel protein sources using enteroids |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
This study was designed to address key questions concerning the use of alternative protein sources for animal feeds and addresses aspects such as their nutrient composition and impact on gut function. We used casein (CAS), spray dried porcine plasma (SDPP), soybean meal (SBM), and yellow meal worm (YMW) as protein sources.
We have investigated the use of intestinal organoids as a model to test the effects of different protein sources on the intestinal epithelium. Mouse enteroids were exposed to different undigested protein sources (4% w/v, viz. soybean meal, SBM; casein, CAS; spray dried plasma protein, SDPP; and yellow meal worm, YMW) or DMEM as a control. Microarrays were used to detail the global gene expression.
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| Overall design |
Confluent 2D monolayers generated from mouse 3D enteroids derived of the duodenum were exposed to different undigested protein sources (4% w/v, viz. soybean meal, SBM; casein, CAS; spray dried plasma protein, SDPP; and yellow meal worm, YMW) or DMEM as a control and RNA isolated for genome-wide transcriptomics.
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| Contributor(s) |
Kar SK, Boekschoten M |
| Citation(s) |
32467755 |
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| Submission date |
Apr 21, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Soumya Kanti Kar |
| E-mail(s) |
soumya.kar@wur.nl
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| Organization name |
WUR
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| Department |
Animal Science Group
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| Lab |
Host Microbe Interactomics
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| Street address |
De Elst1
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| City |
Wageningen |
| State/province |
I am not in the U.S. or Canada |
| ZIP/Postal code |
6708WD |
| Country |
Netherlands |
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| Platforms (1) |
| GPL11533 |
[MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version] |
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| Samples (15)
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| GSM2586171 |
YMW, biological rep1 |
| GSM2586172 |
Media blank, biological rep1 |
| GSM2586173 |
SBM, biological rep2 |
| GSM2586174 |
CAS, biological rep2 |
| GSM2586175 |
SDPP, biological rep2 |
| GSM2586176 |
YMW, biological rep2 |
| GSM2586177 |
Media blank, biological rep2 |
| GSM2586178 |
SBM, biological rep3 |
| GSM2586179 |
CAS, biological rep3 |
| GSM2586180 |
SDPP, biological rep3 |
| GSM2586181 |
YMW, biological rep3 |
| GSM2586182 |
Media blank, biological rep3 |
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| Relations |
| BioProject |
PRJNA383808 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE98051_RAW.tar |
66.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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