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Series GSE97925 Query DataSets for GSE97925
Status Public on Jan 17, 2018
Title Western diet triggers NLRP3-dependent persistent functional reprogramming of myeloid cells [RNA-Seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Here we investigated whether sterile triggers of inflammation  induce trained immunity and thereby influence innate immune responses. Western diet (WD) feeding of Ldlr-/- mice induced systemic inflammation, which was undectable in serum soon after mice were shifted back to chow diet (CD). In contrast, myeloid cell responses towards innate stimuli remained broadly augmented. WD induced transcriptomic and epigenomic reprogramming of myeloid progenitor cells, leading to increased proliferation as well as enhanced innate immune and interferon responses towards in vivo LPS challenge. QTL analysis in human monocytes trained with oxidized low-density lipoprotein (oxLDL) and stimulated with LPS suggested inflammasome-mediated trained immunity. Consistently, Nlrp3-/-/Ldlr-/--deficient mice lacked WD-induced systemic inflammation or myeloid progenitor proliferation and reprogramming. Hence, NLRP3 mediates trained immunity following WD and could thereby arbitrate the potentially deleterious effects of trained immunity in inflammatory diseases.
Overall design Examination of GMPs in six different conditions by RNA-seq
Contributor(s) Christ A, Günther P, Lauterbach MA, Scholz C, Händler K, Baßler K, Klee K, Schultze JL, Latz E
Citation(s) 29328911
Submission date Apr 18, 2017
Last update date Jul 18, 2019
Contact name Joachim Schultze
Organization name LIMES (Life and Medical Sciences Center Genomics and Immunoregulation)
Department Genomics and Immunoregulation
Street address Carl-Troll-Strasse 31
City Bonn
State/province NRW
ZIP/Postal code 53115
Country Germany
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (24)
GSM2581125 Sample_1106
GSM2581126 Sample_1109
GSM2581127 Sample_1960
This SubSeries is part of SuperSeries:
GSE97926 Western diet triggers NLRP3-dependent persistent functional reprogramming of myeloid cells
BioProject PRJNA383373
SRA SRP104167

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Supplementary file Size Download File type/resource
GSE97925_Normalized-processed_RNA-seq_data.txt.gz 893.7 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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