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| Status |
Public on Jan 17, 2018 |
| Title |
Western diet triggers NLRP3-dependent persistent functional reprogramming of myeloid cells [RNA-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Here we investigated whether sterile triggers of inflammation induce trained immunity and thereby influence innate immune responses. Western diet (WD) feeding of Ldlr-/- mice induced systemic inflammation, which was undectable in serum soon after mice were shifted back to chow diet (CD). In contrast, myeloid cell responses towards innate stimuli remained broadly augmented. WD induced transcriptomic and epigenomic reprogramming of myeloid progenitor cells, leading to increased proliferation as well as enhanced innate immune and interferon responses towards in vivo LPS challenge. QTL analysis in human monocytes trained with oxidized low-density lipoprotein (oxLDL) and stimulated with LPS suggested inflammasome-mediated trained immunity. Consistently, Nlrp3-/-/Ldlr-/--deficient mice lacked WD-induced systemic inflammation or myeloid progenitor proliferation and reprogramming. Hence, NLRP3 mediates trained immunity following WD and could thereby arbitrate the potentially deleterious effects of trained immunity in inflammatory diseases.
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| |
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| Overall design |
Examination of GMPs in six different conditions by RNA-seq
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| |
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| Contributor(s) |
Christ A, Günther P, Lauterbach MA, Scholz C, Händler K, Baßler K, Klee K, Schultze JL, Latz E |
| Citation(s) |
29328911 |
| |
| Submission date |
Apr 18, 2017 |
| Last update date |
Jul 18, 2019 |
| Contact name |
Joachim Schultze |
| E-mail(s) |
j.schultze@uni-bonn.de
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| Organization name |
LIMES (Life and Medical Sciences Center Genomics and Immunoregulation)
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| Department |
Genomics and Immunoregulation
|
| Street address |
Carl-Troll-Strasse 31
|
| City |
Bonn |
| State/province |
NRW |
| ZIP/Postal code |
53115 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
|
| Samples (24)
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| This SubSeries is part of SuperSeries: |
| GSE97926 |
Western diet triggers NLRP3-dependent persistent functional reprogramming of myeloid cells |
|
| Relations |
| BioProject |
PRJNA383373 |
| SRA |
SRP104167 |
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