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| Status |
Public on Jun 20, 2017 |
| Title |
Assessing the developmental potential of human pluripotent stem cells using Pluritest |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The International Stem Cell Initiative compared three common approaches for assessing pluripotent stem cells (PSC). The formation of teratomas in vivo, or embryoid bodies (EB) in vitro, provide direct tests of differentiation, whereas PluriTest predicts pluripotency through bioinformatic analysis of transcriptomes of undifferentiated cells. Here we studied the starting poulations of pluripotent stem cells using an Illumina HumanHT-12 V4.0 expression beadchip assays
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| Overall design |
Undifferentiated samples from 16 pluripotent stem cell lines, consisting of 10 embryonic stem cell (ES) and six induced pluripotent stem cell (iPS) lines, from four laboratories were analysed by Illumina HumanHT-12 V4.0 expression beadchip array to asess the degree of pluripotency
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| |
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| Contributor(s) |
Müller F, Schuldt B |
| Citation(s) |
29765017 |
| |
| Submission date |
Apr 17, 2017 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Paul Gokhale |
| E-mail(s) |
p.gokhale@sheffield.ac.uk
|
| Phone |
0114 2222352
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| Organization name |
University of Sheffield
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| Department |
Biomedical Science
|
| Street address |
Western Bank
|
| City |
Sheffield |
| State/province |
N/A |
| ZIP/Postal code |
S10 2TN |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (32)
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| This SubSeries is part of SuperSeries: |
| GSE97964 |
Assessing the developmental and malignant potential of human pluripotent stem cells |
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| Relations |
| BioProject |
PRJNA383140 |
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