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| Status |
Public on Apr 16, 2017 |
| Title |
Genome-wide analysis of the altered gene expression in the transgenic mice ectopically expressing the human SRY. [heart] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Analysis of the effects of an ectopically expressed human SRY (hSRY) on gene expression in the mouse heart. We hypothesize that aberrant expression of the human SRY gene could affect the development and physiology of transgenic mice. To test this hypothesis we have established a Cre-LoxP transgene activation system, thereby activating and expressing the hSRY transgene at the single-cell embryonic stage. Such transgenic mice were born of similar sizes as non-transgenic littermates, but retard in postnatal growth and all die within two weeks of age. To determine the molecular changes associated with such phenotypes, we have analyzed the transcriptomes of hearts of pups expressing (hSRY-ON) and not-expressing (hSRY-OFF) the hSRY transgene. The results provide important information demonstrating that ectopic expression of hSRY resulted in altered expression patterns of numerous genes associating with the development of cardiovascular system and pathogenesis of cardiovascular diseases in the heart.
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| Overall design |
Total RNAs isolated from the heart of P6 male hSRY-ON mice was compared to those of age matched male hSRY-OFF mice.
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| Contributor(s) |
Kido T, Lau YC |
| Citation(s) |
28646221 |
| |
| Submission date |
Apr 15, 2017 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Yun-Fai Chris Lau |
| E-mail(s) |
chris.lau@ucsf.edu
|
| Organization name |
University of California, San Francisco
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| Department |
Department of Medicine, VA Medical Center
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| Street address |
4150 Clement Street
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| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94121 |
| Country |
USA |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE97847 |
Genome-wide analysis of the altered gene expression in the transgenic mice ectopically expressing the human SRY. |
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| Relations |
| BioProject |
PRJNA383042 |
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