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| Status |
Public on Aug 17, 2017 |
| Title |
Lin37 is essential for repression of cell-cycle genes in quiescence |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The DREAM complex represses cell-cycle gene transcription in quiescence. To investigate the role of the DREAM component Lin37, we created Lin37 knockout cells by CRISPR/Cas9-nickase. pRTS episomal vectors expressing luciferase or Lin37 were introduced in two independent knockout clones (411-27 and 632-2) to create Lin37 knockout and rescue cells. To exit the cell cycle and to enter quiescence, cells were serum starved for 60h. RNA-seq was applied to identify genes de-regulated by loss of Lin37 during quiescence.
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| |
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| Overall design |
The following samples are included: clone 411-27 – two biological replicates of each Lin37 knockout and rescue cells; clone 632-2 – two biological and one technical replicate for both Lin37 knockout and rescue cells
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| |
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| Contributor(s) |
Mages CS, Wintsche A, Bernhart SH, Muller GA |
| Citation(s) |
28920576 |
| |
| Submission date |
Apr 12, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Gerd A Müller |
| E-mail(s) |
gerd.mueller@medizin.uni-leipzig.de
|
| Phone |
+493419723636
|
| Organization name |
University of Leipzig
|
| Department |
Medical School
|
| Lab |
Molecular Oncology
|
| Street address |
Semmelweisstrasse 14
|
| City |
Leipzig |
| ZIP/Postal code |
04103 |
| Country |
Germany |
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| Platforms (1) |
| GPL16173 |
Illumina HiScanSQ (Mus musculus) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA382716 |
| SRA |
SRP103846 |
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