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| Status |
Public on Jul 05, 2018 |
| Title |
A versatile drug delivery system targeting senescent cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Senescent cells accumulate in many ageing-associated diseases such as pulmonary fibrosis, and targeting these cells has recently emerged as a promising therapeutic approach. Here, we take advantage of the high β-galactosidase activity of senescent cells to design a targeted drug delivery system based on the encapsulation of drugs with galacto-oligosaccharides (GalNP beads). In this experiment we show that gal-encapsulated rhodamine target senescent cells in the context of pulmonary fibrosis in mice.
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| Overall design |
8- to 10-week-old C57BL/6 wild-type mice were intratracheally inoculated with bleomycin at 1.5 U/kg of body weight. Two weeks later mice were i.v. injected with 200 µl of a solution of GalNP beads loaded with rhodamine [GalNP(rho)] at 4 mg/ml, equivalent to 1 mg/kg of deliverable rhodamine. 6 hours later mice were sacrificed and lung cells were analysed by flow cytometry and sorted into Rho+ or Rho- cells, all CD45-CD31-.
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| Contributor(s) |
Rovira M, Muñoz-Espín D, Fustero-Torre C |
| Citation(s) |
30012580 |
| |
| Submission date |
Apr 12, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Manuel Serrano |
| E-mail(s) |
manuel.serrano@irbbarcelona.org
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| Organization name |
IRB
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| Department |
Molecular Medicine
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| Lab |
Cellular Plasticity and Disease
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| Street address |
Parc Científic de Barcelona, C/ Baldiri Reixac 10
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| City |
Barcelona |
| State/province |
Barcelona |
| ZIP/Postal code |
08028 |
| Country |
Spain |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA382665 |
| SRA |
SRP103831 |
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