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| Status |
Public on Apr 01, 2020 |
| Title |
Expression analysis of INO80 complex in HepG2 liver cancer cells [RNA-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We carried out two sets of experiments to understand how the INO80 complex affected gene expression. Expression analysis following depletion of INO80 with two siRNAs to determine INO80 dependent transcription programs. In addition siMCRS1 or siNTG expression analysis with and without treatment with EZH2 inhibitor (EPZ-6438) was performed to determine what genes in HepG2 cells are de-repressed by EZH2 inhibition, and test the hypothesis that changes at INO80 autonomous sites would depdend on expression of other INO80 subunits (MCRS1). We found that de-repression caused by EZH2 inhbition was blunted when cells were treated with an siRNA targeting MCRS1 and that this was especially pronounced at a set of INO80 target genes.
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| Overall design |
HepG2 cells were treated with siRNA against INO80, MCRS1, or with 1uM EPZ-6438 H3K27me3 inhibitor and subjected to RNAseq.
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| Contributor(s) |
Raab J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 05, 2017 |
| Last update date |
Apr 01, 2020 |
| Contact name |
Jesse Raab |
| E-mail(s) |
jesse.r.raab@gmail.com
|
| Organization name |
University of North Carolina Chapel Hill
|
| Department |
Genetics
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| Street address |
120 Mason Farm Rd
|
| City |
Chapel Hill |
| ZIP/Postal code |
27599 |
| Country |
USA |
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| Platforms (2) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (19)
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| This SubSeries is part of SuperSeries: |
| GSE97413 |
INO80 complex assembly is antagonized by PRC2. |
|
| Relations |
| BioProject |
PRJNA381705 |
| SRA |
SRP103083 |
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