|
| Status |
Public on May 16, 2017 |
| Title |
mRNAseq of Huntington's disease and control patient iPSC-derived brain microvascular endothelial cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: The goals of this study are to compare the transcriptomic profile (mRNA-seq) of HD and control patient iPSC-derived brain microvascular endotheial cells to identify alterations in gene expression.
Methods: RNA were isolated from HD and control iPSC-derived brain microvascular endothelial cells. mRNAseq using Illumina TruSeq mRNA PolyA+ v2 lib prep and HiSeq 2500. Statistical difference in mRNA levels were calculated with subsequent GO and pathway analysis.
Results: mRNAseq and statistical analysis revealed differentially expressed genes between HD and control iPSC-derived brain microvascular endothelial cells.
Conclusions: Our study shows differentially expressed genes between HD and control iPSC-derived brain microvascular endothelial cells, and reveals gene networks that are relevant to the mechanism of HD pathogenesis.
|
| |
|
| Overall design |
mRNA profiles of HD and control iPSC-derived brain microvascular endothelial cells were generated by mRNAseq, in duplicate, using Illumina TruSeq mRNA PolyA+ v2 and Illumina HiSeq 2500.
|
| |
|
| Contributor(s) |
Lim RG, Wu J, Thompson LM |
| Citation(s) |
28514657 |
| |
| Submission date |
Mar 27, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Ryan Gar-Lok Lim |
| E-mail(s) |
limrg@uci.edu
|
| Phone |
9164206047
|
| Organization name |
UC Irvine
|
| Department |
MIND
|
| Lab |
Thompson
|
| Street address |
845 Health Sciences Rd.
|
| City |
Irvine |
| State/province |
California |
| ZIP/Postal code |
92697 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA380669 |
| SRA |
SRP102550 |
Less...
More...