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| Status |
Public on Apr 07, 2017 |
| Title |
Role of NRROS in microglia and perivascular macrophages in the brain |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Mice deficient in NRROS exhibit neurological phenotype similar to ALS. Our previous work showed that in phagocytes, NRROS regulates the degradation of Nox2, the enzymatic component of the NADPH oxidase that generates reactive oxygen species. NRROS is expressed in phagocytes, including macrophages, neutrophils and microglia but not neurons. Previous results on RNA-sequencing from WT and NRRO KO CD11b+ population (NGS601) identified perivascular macrophage (pvm)-like expression profile in NRROS KO cells. FACS data revealed a loss of microglia and concomitant gain of pvm-like cells in the brains of NRROS KO mice.
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| Overall design |
To further these findings, we would like to directly compare gene expression between WT microglia (n = 3), WT pvm (n = 3) and NRROS KO pvm (n = 3).
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| Contributor(s) |
Wong K, Hackney JA, Modrusan Z, Ha C, Stinson J, Guilory J, Ouyang W |
| Citation(s) |
28459434 |
| |
| Submission date |
Mar 23, 2017 |
| Last update date |
Jun 11, 2024 |
| Contact name |
Mark McCreary |
| E-mail(s) |
mccrearm@gene.com
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| Phone |
6502254332
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| Organization name |
Genentech
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| Department |
Bioinformatics
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| Street address |
1 DNA Way, MS 93
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| City |
South San Francisco |
| State/province |
California |
| ZIP/Postal code |
94080 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE84148 |
Gene expression of mouse brain, mouse microglia, and mouse perivascular macrophages (pvm) in NRROS WT and NRROS KO animals |
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| Relations |
| BioProject |
PRJNA380301 |
| SRA |
SRP102398 |
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