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| Status |
Public on Mar 21, 2019 |
| Title |
Peripheral and systemic antigens elicit an expandable pool of resident memory CD8+ T cells in the bone marrow |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Bone marrow (BM) has been put forward as a major reservoir for memory CD8+ T cells. In order to fulfill that function, BM should "store" memory CD8+ T cells, which in biological terms would require these "stored" memory cells to be in disequilibrium with the circulatory pool. This issue is a matter of ongoing debate. Here, we unequivocally demonstrate that murine and human BM harbors a population of tissue-resident memory CD8+ T (TRM ) cells. These cells develop against various pathogens, independently of BM infection or local antigen recognition. BM CD8+ TRM cells share a transcriptional program with resident lymphoid cells in other tissues; they are poly-functional cytokine producers and dependent on IL-15, Blimp-1 and Hobit. CD8+ TRM cells reside in the BM parenchyma, but are in close contact with the circulation. Moreover, this pool of resident T cells is not size-restricted and expands upon peripheral antigenic re-challenge. This works extends the role of the BM in the maintenance of CD8+ T cell memory to include the preservation of an expandable reservoir of functional, non-recirculating memory CD8+ T cells, which develop in response to a large variety of peripheral antigens. This article is protected by copyright. All rights reserved.
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| Overall design |
In total 3 sorts were done, and for each sort the 8 T cell populations were isolated from a pool of 6 mice. The quality of the RNA sample of RM T cells of sort 4 was poor and therefore removed from analysis.
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| Contributor(s) |
Pascutti MF, Geerman S, Nota B, Nolte MA |
| Citation(s) |
30891737 |
| |
| Submission date |
Mar 21, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Benjamin Nota |
| E-mail(s) |
benjamin.nota@gmail.com
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| Phone |
+31 (0)20-5123787
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| Organization name |
Sanquin
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| Department |
Research Facilities
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| Street address |
Plesmanlaan 125
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| City |
Amsterdam |
| State/province |
Noord Holland |
| ZIP/Postal code |
1066 CX |
| Country |
Netherlands |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (23)
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| Relations |
| BioProject |
PRJNA379908 |
| SRA |
SRP102214 |
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