|
| Status |
Public on Mar 29, 2017 |
| Title |
Transcription and chromatin profiling in differentiating THP-1 monocytes and THP-1 derived macrophages |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
|
| Summary |
Here we profile nascent transcription, RNA polymerase III occupancy, chromatin accessibility, and H3K27ac levels in THP-1 monocytes and THP-1 derived macrophages after 72 hr exposure to phorbol myristate acetate (PMA).
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| |
|
| Overall design |
Biotin-capture of nascent transcription, ChIP-seq against RNA polymerase III subunit RPC4 (POLR3D), histone H3K27 acetylation (H3K27ac), CCCTC-binding factor (CTCF), and MAF1, and ATAC-seq in THP-1 monocytes and THP-1 derived macrophages after 72 hr exposure to PMA.
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| |
|
| Contributor(s) |
Van Bortle K, Phanstiel D, Snyder M |
| Citation(s) |
28890333 |
| |
| Submission date |
Mar 19, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Kevin Van Bortle |
| Organization name |
Stanford University
|
| Department |
Genetics
|
| Lab |
Michael Snyder
|
| Street address |
3165 Porter Dr
|
| City |
Palo Alto |
| State/province |
CA |
| ZIP/Postal code |
94043 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (40)
|
|
| Relations |
| BioProject |
PRJNA379744 |
| SRA |
SRP102154 |
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