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Series GSE9624 Query DataSets for GSE9624
Status Public on Oct 01, 2008
Title Differential gene expression in omental adipose tissue from obese children
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Characterization of genes associated with adipose tissue is key to understanding the pathogenesis of obesity and developing treatments for this disorder. Differential gene expression in the adipose tissue has been described in adulthood but none studies have been developed on childhood. The purpose of this study was to compare gene expression in omental adipose tissue from obese prepubertal and normal weight children. We selected 5 obese (BMI adjusted for age and sex z score >2) and 6 normal weight children. RNA was extracted from omental adipose tissue biopsies and cRNA was hybridizated on the human genome U133 Plus 2.0 Arrays (Affymetrix®). Microarray experiments were performed for each sample, and selected group of gene expression values were confirmed with real-time RT-PCR in 10 obese and 10 normal weigth prepubertal children. 1276 genes were found to be differentially expressed at P<0.05. Of those differential genes, 201 were upregulated (Fc>2) and 42 were downregulated (Fc<-2). Genes involved in metabolic and signalling pathways were altered in childhood obesity.
Keywords: disease state analysis
 
Overall design Adipose tissue and blood samples were obtained from 27 children, 14 obese (BMI adjusted for age and sex z score > 2) and 13 non obese undergoing appendix surgery. About 400 mg of adipose tissue was taken and immediately immersed in RNAlater solution and stored at -80°C for gene expression analysis. Informed consent was obtained from all patients after the nature of the study was explained, and the experimental design was approved, from an ethical and scientific standpoint, by the Hospital’s Ethical Committee responsible for research.
 
Contributor(s) Aguilera CM, Tofe I, Suarez A, Gomez-Llorente C, Cañete R, Gil A
Citation(s) 25856673
Submission date Nov 16, 2007
Last update date Mar 25, 2019
Contact name Concepcion María Aguilera
E-mail(s) caguiler@ugr.es
Phone +34958248324
Fax +34958248326
Organization name University of Granada
Department Intitute of Nutrition and Food Technology
Lab Biochemistry and Molecular Biology
Street address Ramon y Cajal, 4
City GRANADA
State/province Granada
ZIP/Postal code 18071
Country Spain
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (11)
GSM243215 Adipose tissue_423_control_rep1
GSM243216 Adipose tissue_425_control_rep2
GSM243217 Adipose tissue_426_control_rep3
Relations
BioProject PRJNA103477

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE9624_RAW.tar 49.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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