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| Status |
Public on May 04, 2018 |
| Title |
The RNA-binding protein Sfpq regulates long neuronal genes in transcriptional elongation [SFPQ_CLIP-seq] |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
Mammalian neurons express extra-long pre-mRNAs which are vulnerable to misregualtion of transcriptional elongation or processing but their regulation is still largely unknown. Here we found that RNA-binding protein PSF is specifically expressed in differentiated neuron of embryonic mouse brain and is essential for the expression of extra-long neuronal gene. HITS-CLIP indicated co-transcriptional binding of PSF to the nascent pre-mRNAs. When PSF was disrupted, pre-mRNA level of long genes was down-regulated in the middle of their transcripts toward their 3’ ends. In Pol II ChIP-Seq, decrease of the RNA polymerase II density was observed parallel to pre-mRNAs, indicating that transcriptional elongation was impaired. Loss of PSF caused massive apoptosis in embryonic mouse brains and heterozygous PSF mutant mice displayed schizophrenia-like abnormal behaviors. Gene Ontology analysis demonstrated that PSF-regulated genes have essential functions at the late developmental stage of brains. Our findings indicate that PSF is required for the neuronal development through facilitating the transcriptional elongation of extra-long neuronal genes and dysfunction of PSF could be a cause of neurodevelopmental and psychiatric disorders.
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| Overall design |
Sfpq CLIP-Seq was perfmored using anti-Sfpq antibody on differentiated Neuro2a cells based on eCLIP methods with modifications.
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| Contributor(s) |
Takeuchi A, Denawa M, Iida K, Hagiwara M |
| Citation(s) |
29719248, 32659723 |
| |
| Submission date |
Mar 10, 2017 |
| Last update date |
Jul 20, 2020 |
| Contact name |
Kei IIDA |
| E-mail(s) |
kiida@life.kindai.ac.jp
|
| Organization name |
Kindai University
|
| Street address |
Kowakae 3-4-1
|
| City |
Higashi-Osaka |
| State/province |
Osaka |
| ZIP/Postal code |
5778502 |
| Country |
Japan |
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| Platforms (1) |
| GPL18635 |
Ion Torrent Proton (Mus musculus) |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE60246 |
The RNA-binding protein Sfpq regulates long neuronal genes in transcriptional elongation |
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| Relations |
| BioProject |
PRJNA378773 |
| SRA |
SRP101723 |
