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| Status |
Public on Jun 26, 2017 |
| Title |
Zoledronic acid inhibits NFAT and IL-2 signaling pathways in regulatory T cells and diminishes their suppressive function in patients with metastatic cancer |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In order to identify the processes altered in T regulatory cells (Treg) by Zoledronic acid (ZA), we examined RNA expression by RNA-seq in Treg treated with and without ZA. We identified gene expression alterations in ZA-treated Treg that were essential to Treg function.
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| Overall design |
Human T regulatory cells isolated from healthy donors (n=6) were cultured overnight with IL-2 and OKT3 (anti-CD3) in the presence or absence of ZA. RNA sequencing (Illumina HiSeq2500) was performed to identify differential gene expression induced by ZA treatment of Treg.
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| |
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| Contributor(s) |
Murray S, Sarhan D, Lundqvist A |
| Citation(s) |
28920001 |
| |
| Submission date |
Mar 10, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Anthony Griswold |
| E-mail(s) |
agriswold@med.miami.edu
|
| Organization name |
University of Miami
|
| Street address |
1501 NW 10th Ave, BRB 318
|
| City |
Miami |
| State/province |
FL |
| ZIP/Postal code |
33136 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA378764 |
| SRA |
SRP101719 |
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